| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2031 | Bacillus | TTTGATGCAGCGCACCAT | CATCGCTGGTAACGTTTCGT | 58.32 | 58.93 | 204 | 50 |
100.00%
|
15.48%
|
| Primer 2032 | Bacillus | TGCAGAGGAAGCTGGGTTG | AAGAGGGGTCGCTGCATT | 59.93 | 58.92 | 160 | 50 |
100.00%
|
15.48%
|
| Primer 2033 | Bacillus | GCAGAGGAAGCTGGGTTGT | AAGAGGGGTCGCTGCATT | 59.93 | 58.92 | 159 | 50 |
100.00%
|
14.29%
|
| Primer 2034 | Bacillus | CCCGTATGATGACGAGCCA | CCTGCATGGACCGCATCAT | 59.26 | 60.53 | 204 | 50 |
100.00%
|
14.29%
|
| Primer 2035 | Bacillus | CCCGTATGATGACGAGCCA | CTGCATGGACCGCATCATG | 59.26 | 59.35 | 203 | 50 |
100.00%
|
14.29%
|
| Primer 2036 | Bacillus | TGCGGACCTTCGTATCCTG | TGCAACGTGGTTTTGTCCA | 59.19 | 58.43 | 178 | 50 |
100.00%
|
14.29%
|
| Primer 2037 | Bacillus | CGTGGTGCAGTTGTTGACC | GCGCTTCGTTATCCATACCG | 59.64 | 59.22 | 181 | 50 |
100.00%
|
14.29%
|
| Primer 2038 | Bacillus | ACAGAAGAAGTGCAAGAGCGT | TTTCACTGCGCATTCCCG | 60.20 | 58.74 | 196 | 50 |
100.00%
|
13.10%
|
| Primer 2039 | Bacillus | ACAAGCGGAACAAGCGGT | GAATTGCCATGCGTGCTCT | 60.20 | 59.19 | 191 | 50 |
100.00%
|
15.48%
|
| Primer 2040 | Bacillus | TTCACCAATGGGCTGCCA | AGCTTCGCTTGACCACATTG | 59.47 | 59.12 | 156 | 50 |
100.00%
|
15.48%
|