| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2131 | Bacillus | TGTGCTTGTATGCGATCGAGA | TGGCAGCAGTATTGACCGC | 59.87 | 60.75 | 290 | 48 |
100.00%
|
13.10%
|
| Primer 2132 | Bacillus | AGCGCATCCAAACGACAGA | ACCATTGGTGCTGCGTCT | 60.01 | 59.57 | 262 | 48 |
100.00%
|
15.48%
|
| Primer 2133 | Bacillus | TTGCACTTCCAGAAGCAGGA | GCCAAAGCGCTTGCACAT | 59.53 | 60.05 | 177 | 48 |
100.00%
|
13.10%
|
| Primer 2134 | Bacillus | ACGACGCAACATGGGCA | TCCACGTTCCCAAGCAAGA | 59.93 | 59.17 | 281 | 48 |
100.00%
|
14.29%
|
| Primer 2135 | Bacillus | AACACTGTTCGCCAACGC | ATCAGCGCTACGAACTGCT | 59.29 | 59.49 | 186 | 48 |
100.00%
|
14.29%
|
| Primer 2136 | Bacillus | ACGAAGATGGTACGTTTGGCT | TGCGAATTGTTTCAAGCGC | 60.00 | 58.21 | 285 | 48 |
100.00%
|
15.48%
|
| Primer 2137 | Bacillus | TGGTCTCAACAATCCTCAGGA | ACCAAACGGCATTTGCGC | 58.38 | 60.36 | 168 | 48 |
100.00%
|
13.10%
|
| Primer 2138 | Bacillus | GGCAGCTATTACGTGGGCT | AGGCACCTGCTGTTGAGA | 59.85 | 58.43 | 215 | 48 |
100.00%
|
15.48%
|
| Primer 2139 | Bacillus | CGGCAGAAGTAACGCCAGA | TACGTTGCACCTGCTCGAG | 60.08 | 60.08 | 226 | 48 |
100.00%
|
15.48%
|
| Primer 2140 | Bacillus | TGCAACGACGCTCGATGTA | CCATCAGCTTCAAGTTGCCG | 59.79 | 59.83 | 156 | 48 |
100.00%
|
15.48%
|