| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2181 | Bacillus | TCCTCGAGACAAACGCGT | TCACGACGAAGGTCTCCCT | 58.96 | 59.93 | 189 | 48 |
100.00%
|
15.48%
|
| Primer 2182 | Bacillus | AACGAGCGCATCCAAACG | ACCATTGGTGCTGCGTCT | 59.14 | 59.57 | 266 | 48 |
100.00%
|
15.48%
|
| Primer 2183 | Bacillus | AGTGGACATGCACCTGAGC | TCGCCTGTTCCATTTGTCGT | 60.00 | 60.25 | 155 | 48 |
100.00%
|
14.29%
|
| Primer 2184 | Bacillus | AAACGCACATTGGAGGCAA | AGCTCCCACTTGAATCCCC | 58.58 | 59.00 | 177 | 48 |
100.00%
|
15.48%
|
| Primer 2185 | Bacillus | AAGCTGCTCGTCGTGCAA | ACGTAATGCTTCGCGTGC | 60.28 | 59.22 | 215 | 48 |
100.00%
|
15.48%
|
| Primer 2186 | Bacillus | GCTAGAGCAGGTGCAACGT | GCCCAATCCGCAGCAAATT | 60.37 | 59.78 | 275 | 48 |
100.00%
|
13.10%
|
| Primer 2187 | Bacillus | AGGTATGGCTAGCGATGGC | TCCCCTTCCTTCATCGCAA | 59.33 | 58.62 | 211 | 48 |
100.00%
|
15.48%
|
| Primer 2188 | Bacillus | ATTGACGACGCCAGAAGCA | TTGCCCTGCAAACACCGT | 60.01 | 60.44 | 169 | 48 |
100.00%
|
15.48%
|
| Primer 2189 | Bacillus | TGTTACAGCCGTTCGTCGT | AACGCCAACTGCTGCAAC | 59.64 | 59.59 | 265 | 48 |
100.00%
|
15.48%
|
| Primer 2190 | Bacillus | ACAAGTGCGTGAGATGGCA | ATCGGAACATGCCCTTCGG | 59.93 | 60.15 | 291 | 48 |
100.00%
|
14.29%
|