| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2211 | Bacillus | TCGAGCTCGATGTCCTTGC | TAAACGGCTTGAAGCAGCG | 59.86 | 59.13 | 269 | 47 |
100.00%
|
14.29%
|
| Primer 2212 | Bacillus | GCCGATTACGTTCCCGACA | AATCATCCCTACGCCAGCG | 60.15 | 59.93 | 180 | 47 |
100.00%
|
15.48%
|
| Primer 2213 | Bacillus | ACCGGTGTTATGTGTCGCT | GCTCCAACAACAACGCTCG | 59.33 | 60.08 | 171 | 47 |
100.00%
|
15.48%
|
| Primer 2214 | Bacillus | TGCAAGAGCGGTTGTACGA | ACCAATTACCCCAGCACCG | 59.64 | 60.00 | 199 | 47 |
100.00%
|
14.29%
|
| Primer 2215 | Bacillus | GGGTATGAGCCAGTCTCGA | TGCCGATGCTGCAAAACG | 58.19 | 59.75 | 259 | 47 |
100.00%
|
15.48%
|
| Primer 2216 | Bacillus | GGGTATGAGCCAGTCTCGA | GCCGATGCTGCAAAACGT | 58.19 | 59.74 | 258 | 47 |
100.00%
|
15.48%
|
| Primer 2217 | Bacillus | AGCAGCTCGTCGTGCAAT | ATGCTTCGCGTGCTACCT | 60.05 | 59.42 | 209 | 47 |
100.00%
|
15.48%
|
| Primer 2218 | Bacillus | AGCAGCTCGTCGTGCAAT | CGTAATGCTTCGCGTGCT | 60.05 | 58.92 | 213 | 47 |
100.00%
|
15.48%
|
| Primer 2219 | Bacillus | TGGTACTGCAGAGCGTCCA | CGCCAACTTTCGTAGCAGC | 60.91 | 59.87 | 167 | 47 |
92.86%
|
15.48%
|
| Primer 2220 | Bacillus | ACCACACGTTGACACTGGT | ACGTCCTAGGCGTCCTTTTG | 59.40 | 60.04 | 214 | 47 |
100.00%
|
15.48%
|