| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Bacillus | TTGTCGGTGGTCGTTCTGG | CGCTGTACCACTTACCGCA | 59.93 | 60.08 | 176 | 88 |
100.00%
|
13.10%
|
| Primer 222 | Bacillus | AACTCCTCCTGCTGCTGT | ATGCCCATACTGCGTGCA | 58.10 | 59.73 | 198 | 88 |
100.00%
|
15.48%
|
| Primer 223 | Bacillus | AACTCCTCCTGCTGCTGTTC | ATGCCCATACTGCGTGCA | 59.96 | 59.73 | 198 | 88 |
100.00%
|
15.48%
|
| Primer 224 | Bacillus | AAACTCCTCCTGCTGCTGT | ATGCCCATACTGCGTGCA | 58.85 | 59.73 | 199 | 88 |
100.00%
|
15.48%
|
| Primer 225 | Bacillus | TCCTCCTGCTGCTGTTCTTC | ATGCCCATACTGCGTGCA | 59.68 | 59.73 | 195 | 88 |
100.00%
|
15.48%
|
| Primer 226 | Bacillus | TCCAGTTGTGATTCGTCGCA | CATTGCTTCTTGCGCTTGC | 59.97 | 58.93 | 253 | 88 |
100.00%
|
15.48%
|
| Primer 227 | Bacillus | TGCGGATCGATTCGAGTGC | TCTGCATTCCCTTGCGCA | 60.59 | 59.97 | 204 | 88 |
100.00%
|
15.48%
|
| Primer 228 | Bacillus | CGGATCGATTCGAGTGCGTA | TCTGCATTCCCTTGCGCA | 60.04 | 59.97 | 202 | 88 |
100.00%
|
15.48%
|
| Primer 229 | Bacillus | ACGCCAAGCAAACCTTCG | AGCAATCGCCTCACTGCA | 58.97 | 59.65 | 256 | 88 |
100.00%
|
15.48%
|
| Primer 230 | Bacillus | ACCTTCTGTACGCGAACTCG | TGTCACTTTCAGCATCCGCA | 60.11 | 60.25 | 293 | 88 |
100.00%
|
13.10%
|