| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Bifidobacterium | AACGCCATGCTGACCGAT | AAGGCAGGTTGACGTCCA | 59.73 | 58.76 | 277 | 135 |
100.00%
|
28.13%
|
| Primer 22 | Bifidobacterium | AGCTGTTCGCCAAGCTGA | ATGATCAGACCGACGCCTG | 59.57 | 59.56 | 232 | 132 |
100.00%
|
34.38%
|
| Primer 23 | Bifidobacterium | GTCGATTTTCCCGACGACG | TGCGCACGTCCATGATGT | 59.01 | 60.05 | 190 | 132 |
100.00%
|
37.50%
|
| Primer 24 | Bifidobacterium | TCCCCAATGAGAAGCGCA | TTGGTCTTGGTGCGCTGA | 58.92 | 59.49 | 292 | 132 |
100.00%
|
56.25%
|
| Primer 25 | Bifidobacterium | CACTCGTGCGAAGGAAACG | TTGGTCTTGGTGCGCTGA | 59.51 | 59.49 | 237 | 132 |
100.00%
|
62.50%
|
| Primer 26 | Bifidobacterium | CTGACGGATGAGCAGCTGA | TTGGTCTTGGTGCGCTGA | 59.49 | 59.49 | 176 | 132 |
100.00%
|
56.25%
|
| Primer 27 | Bifidobacterium | AAGCGCATCGTCGAGCAT | TTGGAACCATCGGTCAGGC | 60.20 | 60.00 | 203 | 132 |
100.00%
|
25.00%
|
| Primer 28 | Bifidobacterium | TTTGGTGTGGGTCGCACT | TTGGTCTTGGTGCGCTGA | 59.41 | 59.49 | 251 | 132 |
100.00%
|
40.63%
|
| Primer 29 | Bifidobacterium | TGGGTCGAGCGCAACAAA | ATGTAGGCGTCGTTGTCGG | 60.20 | 60.15 | 230 | 130 |
100.00%
|
37.50%
|
| Primer 30 | Bifidobacterium | AAGCGCATCGTCGAGCAT | CTCGACGGTGACAACGGAT | 60.20 | 59.79 | 285 | 130 |
100.00%
|
31.25%
|