| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bittarella | TCGCCAACCTCAACGACT | TGAAGGGCACGCTGAACA | 58.87 | 59.49 | 240 | 137 |
100.00%
|
100.00%
|
| Primer 2 | Bittarella | TCTCCAACGAGGACCCCAT | TGCACAGCATGGACAGCA | 59.92 | 59.89 | 171 | 120 |
100.00%
|
100.00%
|
| Primer 3 | Bittarella | TCCAACGAGGACCCCATTG | TGCACAGCATGGACAGCA | 59.32 | 59.89 | 169 | 120 |
100.00%
|
100.00%
|
| Primer 4 | Bittarella | ACTTGATCACCCAGCGCA | TGGAGTTGACCACGTGCA | 59.25 | 59.10 | 243 | 120 |
100.00%
|
100.00%
|
| Primer 5 | Bittarella | TGGCATCTGCTGGACATCC | TGGCCACCGTGTTTGACA | 59.77 | 59.73 | 256 | 119 |
100.00%
|
100.00%
|
| Primer 6 | Bittarella | ACTGGCATCTGCTGGACA | TGGCCACCGTGTTTGACA | 58.51 | 59.73 | 258 | 119 |
100.00%
|
100.00%
|
| Primer 7 | Bittarella | TGCATCATCGTCACCCAGG | AACCCCTGCTCGTTGAAGG | 59.78 | 59.93 | 284 | 118 |
100.00%
|
100.00%
|
| Primer 8 | Bittarella | GTGGTCATGTACGACGCCA | TGGCCACCAAAATGCCCA | 60.08 | 60.12 | 205 | 117 |
100.00%
|
100.00%
|
| Primer 9 | Bittarella | CAGGGGAACAGGCCAAAGT | TGGCCACCAAAATGCCCA | 59.85 | 60.12 | 168 | 117 |
100.00%
|
100.00%
|
| Primer 10 | Bittarella | TGGTCATGTACGACGCCA | TGGCCACCAAAATGCCCA | 58.63 | 60.12 | 204 | 117 |
100.00%
|
100.00%
|