| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Blautia | GGCGAAAGACCAGACTCTCC | GCAACTGTCAGTGCTCTCTG | 60.11 | 58.85 | 200 | 107 |
100.00%
|
36.36%
|
| Primer 2 | Blautia | TGCGTTGGTATGGCTGCA | ATCTGTGCCGTCCATCCAG | 59.97 | 59.48 | 248 | 105 |
100.00%
|
36.36%
|
| Primer 3 | Blautia | TGCGTTGGTATGGCTGCA | AGATCTGTGCCGTCCATCC | 59.97 | 59.17 | 250 | 105 |
100.00%
|
36.36%
|
| Primer 4 | Blautia | TCCGGTTATCCAGAGCGTG | CCACAGAGCGGTCGTATGT | 59.19 | 59.49 | 181 | 88 |
100.00%
|
36.36%
|
| Primer 5 | Blautia | TCCGGTTATCCAGAGCGTG | TTTTGCCACAGAGCGGTC | 59.19 | 58.26 | 186 | 85 |
100.00%
|
36.36%
|
| Primer 6 | Blautia | TCGGAGGGACTTGTTTTGCA | TCTGCCCACCTGCAAGTTC | 59.82 | 60.23 | 190 | 80 |
100.00%
|
36.36%
|
| Primer 7 | Blautia | AGGTGCGAAGAGAGCAGAC | ACCTGTTTCTTATCGCGTCCT | 59.41 | 59.45 | 174 | 80 |
100.00%
|
36.36%
|
| Primer 8 | Blautia | CCGGTTATCCAGAGCGTGA | CCACAGAGCGGTCGTATGT | 59.19 | 59.49 | 180 | 72 |
100.00%
|
36.36%
|
| Primer 9 | Blautia | TGTTGTAGGCGACGGCAA | TCCAGTACCTTCCGGAGCT | 59.58 | 59.61 | 205 | 70 |
100.00%
|
36.36%
|
| Primer 10 | Blautia | TTGTTGTAGGCGACGGCA | TCCAGTACCTTCCGGAGCT | 59.58 | 59.61 | 206 | 70 |
100.00%
|
36.36%
|