| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 261 | Brachyspira | GTGTCTGCAGATGACGGTAC | GCAGCCTTCCCCTCCATTT | 58.37 | 60.00 | 206 | 77 |
100.00%
|
50.00%
|
| Primer 262 | Brachyspira | ACCGCAGCAGGGAATAGT | GCCACTCTTTGCTGCTGAC | 58.27 | 59.42 | 207 | 77 |
100.00%
|
50.00%
|
| Primer 263 | Brachyspira | GAATTCGGCTGAGGCTGTTT | GCAGGCAAAGTCATAACTCCG | 58.83 | 59.60 | 212 | 77 |
100.00%
|
75.00%
|
| Primer 264 | Brachyspira | AGAAATAGGCTGCGGAAATGG | TGGCCAAGGATCTGGAAAGT | 58.70 | 58.92 | 241 | 76 |
100.00%
|
50.00%
|
| Primer 265 | Brachyspira | AGTTGGCAAACCTTTAGCTCA | ACAGTAACACTTCCAACCCCA | 58.06 | 59.15 | 294 | 76 |
100.00%
|
50.00%
|
| Primer 266 | Brachyspira | AGCGCTTTACCTGGTACACA | GCACCTAAAACACCGCTGA | 59.32 | 58.38 | 299 | 76 |
100.00%
|
75.00%
|
| Primer 267 | Brachyspira | GCCTGTTGATGTAAGACCGGA | AAGTGTGCGAACGCTTTGT | 60.07 | 58.91 | 201 | 76 |
100.00%
|
50.00%
|
| Primer 268 | Brachyspira | AGCGATGACAGCTGGGTTC | TGCTTGCTGTAGGATACCCC | 60.08 | 59.16 | 151 | 76 |
100.00%
|
50.00%
|
| Primer 269 | Brachyspira | ACCTGGTGCTGCTACTCCT | CCAAGTACCTCCGCATGCA | 60.23 | 60.08 | 226 | 76 |
100.00%
|
50.00%
|
| Primer 270 | Brachyspira | AGCGGAATAGACGGCTCAC | ACTTCATCGCGTACTGGCA | 59.56 | 59.41 | 203 | 76 |
100.00%
|
50.00%
|