| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 581 | Brachyspira | TGGACCATTTGCATCCGCT | ACGTGAAGCTCAACAGTTCTT | 60.00 | 58.09 | 172 | 56 |
100.00%
|
50.00%
|
| Primer 582 | Brachyspira | GCAGCAGGTTTTCTCACGC | TAGAATGATGCCACCACCCC | 60.08 | 59.45 | 153 | 56 |
100.00%
|
50.00%
|
| Primer 583 | Brachyspira | AGCGTGCTCAGCTTGGTA | TGCCCTTGCTATCTCGCA | 58.94 | 58.69 | 233 | 56 |
100.00%
|
50.00%
|
| Primer 584 | Brachyspira | TGCGAGATAGCAAGGGCA | ACGTGCCAAAGGATTCGC | 58.69 | 58.74 | 258 | 56 |
100.00%
|
50.00%
|
| Primer 585 | Brachyspira | ACGCAAATTCGCTGATGGTG | ACGCCAGAAGCTTGCAAGA | 59.83 | 60.23 | 270 | 56 |
100.00%
|
50.00%
|
| Primer 586 | Brachyspira | GCTGCTGATGAGGCTCAGA | GCCCTCATCATAGCCTTTAGC | 59.48 | 58.57 | 225 | 56 |
100.00%
|
50.00%
|
| Primer 587 | Brachyspira | CCATTGCCTACAAGCATACGT | GCTGAACATCAACCCCAGC | 58.71 | 59.11 | 178 | 55 |
100.00%
|
50.00%
|
| Primer 588 | Brachyspira | CCATTGCCTACAAGCATACGT | ACATCAACCCCAGCTGGA | 58.71 | 58.08 | 173 | 55 |
100.00%
|
50.00%
|
| Primer 589 | Brachyspira | CCATTGCCTACAAGCATACGT | GAACATCAACCCCAGCTGGA | 58.71 | 59.96 | 175 | 55 |
100.00%
|
50.00%
|
| Primer 590 | Brachyspira | CCATTGCCTACAAGCATACGT | ACATCAACCCCAGCTGGAAG | 58.71 | 59.96 | 173 | 55 |
100.00%
|
50.00%
|