| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Brevibacillus | GCAAAGTGGCGAGTTGTCC | TGCTTTCGCCTTGCTTGG | 59.72 | 58.96 | 176 | 152 |
100.00%
|
50.00%
|
| Primer 2 | Brevibacillus | AGTGCGCAACCAAAGTGC | AGCAAGACGAACGGCAAAC | 59.59 | 59.35 | 201 | 141 |
100.00%
|
50.00%
|
| Primer 3 | Brevibacillus | CACGCGTATGGGCAAATGG | TTCATTGCGCAAGGCTGC | 59.94 | 59.74 | 254 | 138 |
100.00%
|
50.00%
|
| Primer 4 | Brevibacillus | TCCGTGGGGTCATGCATTC | TTCATTGCGCAAGGCTGC | 60.08 | 59.74 | 164 | 138 |
100.00%
|
50.00%
|
| Primer 5 | Brevibacillus | ATGCAGGCGTACTCGTTCC | AATGCCTGGCGTCGATGT | 60.15 | 59.73 | 185 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Brevibacillus | GCAAAGTGGCGAGTTGTCC | ATGCTTTCGCCTTGCTTGG | 59.72 | 59.41 | 177 | 132 |
100.00%
|
50.00%
|
| Primer 7 | Brevibacillus | TCGATTGGGACGCATGGAG | AGGAGAGAACGGAGGCGAT | 59.86 | 60.08 | 248 | 131 |
100.00%
|
50.00%
|
| Primer 8 | Brevibacillus | AGTGCGCAACCAAAGTGC | AGACGAACGGCAAACAGGA | 59.59 | 59.56 | 197 | 130 |
100.00%
|
50.00%
|
| Primer 9 | Brevibacillus | TTGCTTGCGGTGCTTGTG | GGGCATCCTTCAGCAGCAT | 59.59 | 60.46 | 151 | 129 |
100.00%
|
50.00%
|
| Primer 10 | Brevibacillus | TGGTGCTGGTTGCTCTGT | TGAATCGCAAGCAACGCC | 59.08 | 59.44 | 199 | 128 |
100.00%
|
50.00%
|