| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Brevibacillus | TGGAACAGGCTTGCGTGT | TGCGGTCTCATTGCAGACA | 59.81 | 59.63 | 265 | 127 |
100.00%
|
50.00%
|
| Primer 22 | Brevibacillus | GCAAGGCGTTAGAGATTGAGG | TCGGCTCTTTCTTCCCAGG | 59.06 | 59.02 | 178 | 127 |
100.00%
|
50.00%
|
| Primer 23 | Brevibacillus | GCAAGGCGTTAGAGATTGAGG | TTCGGCTCTTTCTTCCCAGG | 59.06 | 59.68 | 179 | 127 |
100.00%
|
50.00%
|
| Primer 24 | Brevibacillus | ACTGGACCAAACGCTCTGG | AACGTTGCGCATTGGAGC | 59.93 | 59.74 | 222 | 126 |
100.00%
|
50.00%
|
| Primer 25 | Brevibacillus | AGTGCGCAACCAAAGTGC | GTGTTGGGGTAGTCTGCCA | 59.59 | 59.24 | 271 | 125 |
100.00%
|
50.00%
|
| Primer 26 | Brevibacillus | TGCTGCCGTATTTGCCCA | ACGCTTCCTCCACGTCTTC | 59.97 | 59.71 | 248 | 125 |
100.00%
|
50.00%
|
| Primer 27 | Brevibacillus | TCACCATGCAGCTTGCCA | CGGAAACAAGGCCAGCTTG | 59.88 | 59.71 | 200 | 125 |
100.00%
|
50.00%
|
| Primer 28 | Brevibacillus | TTGCCGATGCGTGATCCA | GGTTTTGCAGGTACGCGAG | 59.73 | 59.50 | 154 | 125 |
100.00%
|
50.00%
|
| Primer 29 | Brevibacillus | ACACGGTCGTCTTTGGCA | ACAGGCGAACGAACTCCA | 59.50 | 58.87 | 255 | 125 |
100.00%
|
50.00%
|
| Primer 30 | Brevibacillus | ACACGGTCGTCTTTGGCA | AACAGGCGAACGAACTCCA | 59.50 | 59.56 | 256 | 125 |
100.00%
|
50.00%
|