| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2541 | Brevibacillus | TCGGTGCGTTATTGGCGT | TTGGCTACCCGATCCTCGA | 60.05 | 60.08 | 187 | 59 |
100.00%
|
50.00%
|
| Primer 2542 | Brevibacillus | TTTGGCCTGGTACACGCA | GTCACATCCAAGCCGACGA | 59.49 | 60.08 | 213 | 59 |
100.00%
|
50.00%
|
| Primer 2543 | Brevibacillus | TTTGGCTTGGGACAGCGA | TCAACGAGTTCGCGACCAA | 59.49 | 59.93 | 156 | 59 |
100.00%
|
50.00%
|
| Primer 2544 | Brevibacillus | TTTGGCTTGGGACAGCGA | TTCAACGAGTTCGCGACCA | 59.49 | 59.93 | 157 | 59 |
100.00%
|
50.00%
|
| Primer 2545 | Brevibacillus | TTTGGCTTGGGACAGCGA | GCTTCAACGAGTTCGCGAC | 59.49 | 59.87 | 159 | 59 |
100.00%
|
50.00%
|
| Primer 2546 | Brevibacillus | TTTGGCTTGGGACAGCGA | TGCTTCAACGAGTTCGCG | 59.49 | 58.77 | 160 | 59 |
100.00%
|
50.00%
|
| Primer 2547 | Brevibacillus | TGAACCTCTCTGCACCCGA | TGCAGCTTGCCTTGGGAA | 60.53 | 59.80 | 286 | 59 |
100.00%
|
50.00%
|
| Primer 2548 | Brevibacillus | TGAACCTCTCTGCACCCGA | TTGCAGCTTGCCTTGGGA | 60.53 | 59.80 | 287 | 59 |
100.00%
|
50.00%
|
| Primer 2549 | Brevibacillus | ACCGATGCAGCTGACCAA | AATGCTCAACGGCACCGA | 59.25 | 59.97 | 200 | 59 |
100.00%
|
50.00%
|
| Primer 2550 | Brevibacillus | AACCGATGCAGCTGACCA | AATGCTCAACGGCACCGA | 59.25 | 59.97 | 201 | 59 |
100.00%
|
50.00%
|