| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3491 | Brevibacillus | GCAAATCCCTGCACACCTT | ACCATTTGACCAGAAACGGGA | 58.65 | 59.86 | 169 | 52 |
100.00%
|
50.00%
|
| Primer 3492 | Brevibacillus | GCAAATCCCTGCACACCTT | GACCAGAAACGGGAATCTGCT | 58.65 | 60.34 | 162 | 52 |
100.00%
|
50.00%
|
| Primer 3493 | Brevibacillus | ACCGTGTGGAGAATGACGC | TGCCAAAATGCGCATGCT | 60.37 | 59.34 | 228 | 52 |
100.00%
|
50.00%
|
| Primer 3494 | Brevibacillus | ATTTCGTTTGCGCTGGGC | TGGCATGGGGAAACAGCA | 59.74 | 59.47 | 235 | 52 |
100.00%
|
50.00%
|
| Primer 3495 | Brevibacillus | TCGATTGGGACGCATGGAG | ACGGAGGCGATAACAGGAC | 59.86 | 59.19 | 240 | 51 |
100.00%
|
50.00%
|
| Primer 3496 | Brevibacillus | TGCGAATTGTGTGCCGAC | TAAGCGCTGCTCCGTGAT | 59.06 | 59.11 | 298 | 51 |
100.00%
|
50.00%
|
| Primer 3497 | Brevibacillus | TGGTTCATGCGTCGGTGT | TCTTTGCAGGCGTCGACA | 59.58 | 59.58 | 279 | 51 |
100.00%
|
50.00%
|
| Primer 3498 | Brevibacillus | AGGGATATCGCCATGCACG | AACGCTTTCAGGCTGGCT | 60.00 | 59.89 | 269 | 51 |
100.00%
|
50.00%
|
| Primer 3499 | Brevibacillus | TTTGTCCAAGCCAGCGGT | AATGGCGAGCGACGAACT | 59.81 | 59.74 | 212 | 51 |
100.00%
|
50.00%
|
| Primer 3500 | Brevibacillus | GCACGCAAAACTCGCTGA | AGGTAGCCCTTGTCCTGGT | 59.37 | 59.84 | 180 | 51 |
100.00%
|
50.00%
|