| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4821 | Brevibacillus | ACAACGCTGCTCCGCTAT | TTGTCGAGGCGATACCCCA | 59.42 | 60.68 | 194 | 42 |
100.00%
|
50.00%
|
| Primer 4822 | Brevibacillus | AGCCGTAGCGACTTTGGT | TCGCAGCCGTCACATACA | 58.94 | 59.03 | 186 | 42 |
100.00%
|
50.00%
|
| Primer 4823 | Brevibacillus | TGGCGTTGACGATGCTGT | GATCGCCTTGTCGGAGGAA | 59.97 | 59.48 | 169 | 42 |
100.00%
|
50.00%
|
| Primer 4824 | Brevibacillus | CAAAAGGATTGGCGTGCCC | CGTGCTTCATCATTTCGTGGT | 60.08 | 59.54 | 159 | 42 |
100.00%
|
50.00%
|
| Primer 4825 | Brevibacillus | ACCGCTCTTTGTTCCGCA | TGTTGGTACGCTTGCAGGT | 59.89 | 59.85 | 186 | 42 |
100.00%
|
50.00%
|
| Primer 4826 | Brevibacillus | AGTCAATGCTGCGCTGGA | AAAACTCCGCTGGACGCT | 59.65 | 59.57 | 260 | 42 |
100.00%
|
50.00%
|
| Primer 4827 | Brevibacillus | TCCAGTCAATGCTGCGCT | AAAACTCCGCTGGACGCT | 59.65 | 59.57 | 263 | 42 |
100.00%
|
50.00%
|
| Primer 4828 | Brevibacillus | TTGGCTGTCGAACCGGAA | TCCAGCAAATCGTCGGCA | 59.18 | 59.65 | 209 | 42 |
100.00%
|
50.00%
|
| Primer 4829 | Brevibacillus | ACGCATCGCTTGGGAGAA | AATACGGCAGCGTCAGGT | 59.34 | 59.02 | 233 | 42 |
100.00%
|
50.00%
|
| Primer 4830 | Brevibacillus | TTTGGTGTGCCGACGGAT | ATCGCTGCATCAGTCGGT | 59.57 | 59.10 | 226 | 42 |
100.00%
|
50.00%
|