| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 5131 | Brevibacillus | TTGTCCAGCCAGCACGTT | ACCCCTTTCCACACCTCCT | 59.81 | 60.07 | 275 | 40 |
100.00%
|
50.00%
|
| Primer 5132 | Brevibacillus | TGCCGTCATTCGCATGGA | TAAACCACGCGTTCGCCT | 59.73 | 59.97 | 176 | 40 |
100.00%
|
50.00%
|
| Primer 5133 | Brevibacillus | AGCGGACGATCCTGCAAA | AACAATCGCCACTCGCCA | 59.34 | 59.97 | 241 | 40 |
100.00%
|
50.00%
|
| Primer 5134 | Brevibacillus | AGCGATTGACCGGTCGTT | TTTCGCAGCAGGCCTTGA | 59.35 | 59.89 | 217 | 40 |
100.00%
|
50.00%
|
| Primer 5135 | Brevibacillus | AGCGATTGACCGGTCGTT | TCCGCAAACACAACCGGA | 59.35 | 59.81 | 256 | 40 |
100.00%
|
50.00%
|
| Primer 5136 | Brevibacillus | AGCGATTGACCGGTCGTT | TGTTTCGCAGCAGGCCTT | 59.35 | 60.20 | 219 | 40 |
100.00%
|
50.00%
|
| Primer 5137 | Brevibacillus | AGCGCTGGCGATTCTCAA | TGATGTGGCTGGTCCGTT | 59.73 | 58.84 | 218 | 40 |
100.00%
|
50.00%
|
| Primer 5138 | Brevibacillus | AAGCGCTGGCGATTCTCA | TGATGTGGCTGGTCCGTT | 59.73 | 58.84 | 219 | 40 |
100.00%
|
50.00%
|
| Primer 5139 | Brevibacillus | CTCGTGGACGGCTACAACA | TGCTTCTGCTTGCGACCA | 59.71 | 59.89 | 188 | 40 |
100.00%
|
50.00%
|
| Primer 5140 | Brevibacillus | ACGATGCTGCAAGGCCTT | AGCATCGCGTCATCCGTT | 59.97 | 59.82 | 262 | 40 |
100.00%
|
50.00%
|