| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 5751 | Brevibacillus | TGGGTGTTGAAGTGGGCAG | CACATCCGGACGGTCGATT | 60.15 | 59.86 | 242 | 35 |
100.00%
|
50.00%
|
| Primer 5752 | Brevibacillus | ACCACCACATCAACGGCA | AATCGTCCGCTTGGTCGT | 59.49 | 59.35 | 200 | 35 |
100.00%
|
50.00%
|
| Primer 5753 | Brevibacillus | TGCCGAAGTGCTGGTCAA | ACCGCTTGATGTGCTCCA | 59.49 | 59.25 | 176 | 35 |
100.00%
|
50.00%
|
| Primer 5754 | Brevibacillus | ATCGACAGCGCAGTGGTT | TCTCGTTGGCAATCGCGT | 59.66 | 60.05 | 277 | 35 |
100.00%
|
50.00%
|
| Primer 5755 | Brevibacillus | AATCGACAGCGCAGTGGT | TCTCGTTGGCAATCGCGT | 59.66 | 60.05 | 278 | 35 |
100.00%
|
50.00%
|
| Primer 5756 | Brevibacillus | CTATGCAGGGTCGTGTCGT | CCTTGGAAAGCGGACGAGT | 59.49 | 60.00 | 189 | 35 |
100.00%
|
50.00%
|
| Primer 5757 | Brevibacillus | TGCCAGCAGAAACACCACA | GCCTTCTGACAATCTGCCG | 60.08 | 58.91 | 161 | 35 |
100.00%
|
50.00%
|
| Primer 5758 | Brevibacillus | GGCAAGCGCATCCGAATT | CTTCACGCAGGTGGCAGTA | 59.20 | 60.01 | 214 | 35 |
100.00%
|
50.00%
|
| Primer 5759 | Brevibacillus | GGCAAGCGCATCCGAATT | GCAGGTGGCAGTAACCGAT | 59.20 | 60.08 | 208 | 35 |
100.00%
|
50.00%
|
| Primer 5760 | Brevibacillus | TTTCACCTGTCGTCGGCT | AGTCGCGATCAAACCCGT | 58.87 | 59.35 | 180 | 35 |
100.00%
|
50.00%
|