| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Butyricicoccus | AACCGATGGCATGCGTGT | TGCATCGTCGCGAATCTCA | 60.36 | 59.86 | 253 | 132 |
100.00%
|
100.00%
|
| Primer 42 | Butyricicoccus | AGTGATGCCGACGTACTGC | TGGTTGCCTTGACGCTGA | 60.15 | 59.49 | 295 | 132 |
100.00%
|
100.00%
|
| Primer 43 | Butyricicoccus | TGATGCCGACGTACTGCTG | TGGTTGCCTTGACGCTGA | 60.15 | 59.49 | 293 | 132 |
100.00%
|
100.00%
|
| Primer 44 | Butyricicoccus | ACAATGCGTTTGCCGACG | GACACCGCATCCCAGTTCA | 59.75 | 60.00 | 176 | 132 |
100.00%
|
100.00%
|
| Primer 45 | Butyricicoccus | ACAATGCGTTTGCCGACG | CTGCACGCAGCCAATACAC | 59.75 | 59.86 | 276 | 132 |
100.00%
|
100.00%
|
| Primer 46 | Butyricicoccus | ACAATGCGTTTGCCGACG | ACACCGCATCCCAGTTCA | 59.75 | 58.84 | 175 | 132 |
100.00%
|
100.00%
|
| Primer 47 | Butyricicoccus | CTGCGGTCTGTCACAGAGT | TGTGCGCAGAAAAGCAGC | 59.34 | 59.67 | 223 | 132 |
100.00%
|
100.00%
|
| Primer 48 | Butyricicoccus | CAGGCGTGATGGAGCTTGA | AGCTCCTGCCAGTTTGCA | 60.08 | 59.48 | 223 | 132 |
100.00%
|
100.00%
|
| Primer 49 | Butyricicoccus | GCGCTGAAGGATCTGGTGA | AGCTCCTGCCAGTTTGCA | 59.78 | 59.48 | 269 | 132 |
100.00%
|
100.00%
|
| Primer 50 | Butyricicoccus | AGGCGTGATGGAGCTTGA | AGCTCCTGCCAGTTTGCA | 58.61 | 59.48 | 222 | 132 |
100.00%
|
100.00%
|