| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Caldicellulosiruptor | TGAACGACATGCAGCTTGC | TTCCAGTTGGCTCATCGGC | 59.42 | 60.38 | 297 | 51 |
100.00%
|
50.00%
|
| Primer 222 | Caldicellulosiruptor | GGTGTCTACACTTTCTTCGGC | GCAAGCTCAAGGTCAGCAAG | 58.93 | 59.76 | 167 | 51 |
100.00%
|
50.00%
|
| Primer 223 | Caldicellulosiruptor | GGTGTCTACACTTTCTTCGGC | GCTCAAGGTCAGCAAGAGGA | 58.93 | 59.68 | 163 | 51 |
100.00%
|
50.00%
|
| Primer 224 | Caldicellulosiruptor | ACACATGGGGTTGCTGCA | TGGATACTGGGCTGGTTTGT | 59.80 | 58.93 | 233 | 50 |
100.00%
|
50.00%
|
| Primer 225 | Caldicellulosiruptor | TGCAGTGTTTGGAGTTGGC | TCCGCAAACAGGGCAGAT | 58.89 | 59.24 | 256 | 50 |
100.00%
|
50.00%
|
| Primer 226 | Caldicellulosiruptor | GCAGTGTTTGGAGTTGGCAC | TCCGCAAACAGGGCAGAT | 60.25 | 59.24 | 255 | 50 |
100.00%
|
50.00%
|
| Primer 227 | Caldicellulosiruptor | TCAAACTGGTGCTGGGCA | CGGCAAAATGTCCGGTTCC | 59.40 | 59.79 | 298 | 50 |
100.00%
|
50.00%
|
| Primer 228 | Caldicellulosiruptor | TGGCGAGTGGGAAGGTTTG | TGCATTCCCAATCCAGCACT | 60.23 | 59.96 | 274 | 50 |
100.00%
|
50.00%
|
| Primer 229 | Caldicellulosiruptor | TGGCAAACGCTGTTGAAAATG | TGCGCAACTGTTTCGAGC | 58.80 | 59.37 | 244 | 50 |
100.00%
|
50.00%
|
| Primer 230 | Caldicellulosiruptor | TGGCAAACGCTGTTGAAAATG | ATGCGCAACTGTTTCGAGC | 58.80 | 59.79 | 245 | 50 |
100.00%
|
50.00%
|