| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 511 | Caldicellulosiruptor | ACATCCCATGGCAGGCA | TCACAAGCCCTGCAGACA | 58.45 | 58.76 | 236 | 45 |
100.00%
|
50.00%
|
| Primer 512 | Caldicellulosiruptor | TGGCAAAAGTTGGTTGGGGA | TGTATTGCCTCACCCTCAGG | 60.33 | 59.09 | 265 | 45 |
100.00%
|
50.00%
|
| Primer 513 | Caldicellulosiruptor | AGAGGGATGAAGTGGGCAAA | GCCAACCCTTCTTGTTGCA | 58.92 | 58.88 | 252 | 45 |
100.00%
|
50.00%
|
| Primer 514 | Caldicellulosiruptor | AGAGGGATGAAGTGGGCAAA | TGCCAACCCTTCTTGTTGC | 58.92 | 58.88 | 253 | 45 |
100.00%
|
50.00%
|
| Primer 515 | Caldicellulosiruptor | GGCGTTGGGTTTACACGGA | AGCGCACTTCCAGAGGAT | 60.60 | 58.28 | 178 | 45 |
100.00%
|
100.00%
|
| Primer 516 | Caldicellulosiruptor | AGACATTTGGAGGGGTGCA | TCTGAGAGCTCCCAGGACA | 58.84 | 59.22 | 248 | 45 |
100.00%
|
50.00%
|
| Primer 517 | Caldicellulosiruptor | AGACGCGACCCATCCAAA | AACAAGGTCGTACGGCAGG | 58.93 | 60.01 | 165 | 45 |
100.00%
|
50.00%
|
| Primer 518 | Caldicellulosiruptor | GGCGAGCTTGAAGTCAGTG | TGCAACGTCTTGAGCAGAAG | 58.84 | 58.78 | 246 | 45 |
100.00%
|
50.00%
|
| Primer 519 | Caldicellulosiruptor | CACACCTGATGAATGGCAGAG | TTTGTGACCCTGTCTGCCC | 58.99 | 59.85 | 200 | 45 |
100.00%
|
50.00%
|
| Primer 520 | Caldicellulosiruptor | AAGGGTTGCTGTTGCTGC | TGCCGAGCAAGCTCAACA | 58.87 | 59.89 | 225 | 45 |
100.00%
|
50.00%
|