| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Chlamydia | TGACCACTACGCGTGATCC | GCTACTCCAGCGACTTCTCC | 59.49 | 59.90 | 214 | 149 |
100.00%
|
52.94%
|
| Primer 2 | Chlamydia | GTCGCGATATAGAGACCCGTG | TGAACCGCAGCAACATGC | 60.14 | 59.36 | 289 | 146 |
100.00%
|
47.06%
|
| Primer 3 | Chlamydia | TCGCGATATAGAGACCCGTG | TGAACCGCAGCAACATGC | 58.85 | 59.36 | 288 | 146 |
100.00%
|
47.06%
|
| Primer 4 | Chlamydia | TGATGGCTTCAGTGGCTGG | AAACCCCTTCGCTGTCAGG | 60.00 | 59.93 | 272 | 145 |
100.00%
|
47.06%
|
| Primer 5 | Chlamydia | AGCTTCCACTGCAAGAGGC | CGACAGTAAGTTCTTGGCCAG | 60.30 | 58.93 | 182 | 143 |
100.00%
|
52.94%
|
| Primer 6 | Chlamydia | ATGGCAGATCTCAGCGCTC | ACGCTTTCCTGCCTGTACC | 59.93 | 60.00 | 237 | 138 |
100.00%
|
47.06%
|
| Primer 7 | Chlamydia | TTTCTCCGCTGGTAGGCAC | ACGCGACCAGACATTCCTG | 59.70 | 60.08 | 154 | 136 |
100.00%
|
47.06%
|
| Primer 8 | Chlamydia | AGCTTCCACTGCAAGAGGC | TCGACAGTAAGTTCTTGGCCA | 60.30 | 59.31 | 183 | 135 |
100.00%
|
52.94%
|
| Primer 9 | Chlamydia | AGGGGTGTTGGTAGCTCCA | CCGCTGCCTCAAAATGCA | 60.15 | 59.04 | 281 | 135 |
100.00%
|
47.06%
|
| Primer 10 | Chlamydia | TGGCGAAGAAGTCAGCAGT | ACGCCAGGAATATCTCCCAT | 59.25 | 58.27 | 286 | 132 |
100.00%
|
58.82%
|