| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Cloacibacillus | TCGACATGCTGCGGAACA | AGTCGATGAAGGCCTGCTG | 59.66 | 59.78 | 210 | 99 |
100.00%
|
50.00%
|
| Primer 52 | Cloacibacillus | TTTCGAGCGTGAGGGCAA | AGCCCTCCTTGACCTCCAT | 59.58 | 59.92 | 170 | 99 |
100.00%
|
50.00%
|
| Primer 53 | Cloacibacillus | ACGCAAACGATCTCGGCT | TTGATCATCGCCTCGTCCG | 59.74 | 59.93 | 151 | 97 |
100.00%
|
50.00%
|
| Primer 54 | Cloacibacillus | ACGCAAACGATCTCGGCT | TTGATGTCCGTGCAGAGGC | 59.74 | 60.38 | 193 | 97 |
100.00%
|
50.00%
|
| Primer 55 | Cloacibacillus | ATTTCCTGACGCTGCGCT | GCATACCCTCCTGCGTGAA | 60.05 | 59.78 | 282 | 97 |
100.00%
|
50.00%
|
| Primer 56 | Cloacibacillus | ATTTCCTGACGCTGCGCT | TACGGCTGGCATTTCTCCC | 60.05 | 59.78 | 157 | 97 |
100.00%
|
50.00%
|
| Primer 57 | Cloacibacillus | ATTTCCTGACGCTGCGCT | ATACCCTCCTGCGTGAACC | 60.05 | 59.09 | 280 | 97 |
100.00%
|
50.00%
|
| Primer 58 | Cloacibacillus | CGCTGCAGAGGGAGTTCAT | TTCGCCTGCCTTTCGAGT | 59.78 | 59.26 | 217 | 97 |
100.00%
|
50.00%
|
| Primer 59 | Cloacibacillus | ACGCATCTGCTTCGAGCT | TGCGCTGCAAGATGCTCA | 59.42 | 60.36 | 239 | 97 |
100.00%
|
100.00%
|
| Primer 60 | Cloacibacillus | TCGGCTTTGTCAACCGCT | CGCTGAAGGCCTGGATCAA | 59.89 | 60.08 | 282 | 97 |
100.00%
|
50.00%
|