| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 171 | Clostridioides | AGGGGTGCAAAGTCTGATGT | TGAGCAGCCACTTTATCAGCA | 59.23 | 60.00 | 254 | 71 |
100.00%
|
61.54%
|
| Primer 172 | Clostridioides | TTGACTGATGCAGGTTCACC | CCTACCCAACTGTATGCCCAT | 58.10 | 59.51 | 188 | 71 |
100.00%
|
61.54%
|
| Primer 173 | Clostridioides | AGTTTGTGGTTCAGGGACAGT | TCGCCTGTTTGAAGTGGACT | 59.44 | 59.53 | 177 | 71 |
100.00%
|
69.23%
|
| Primer 174 | Clostridioides | TGTGATGAACCTGTTTCTGCA | TTGGTACTGCGCTCATAAGC | 58.07 | 58.35 | 247 | 71 |
100.00%
|
61.54%
|
| Primer 175 | Clostridioides | TGTGATGAACCTGTTTCTGCA | ATTGGTACTGCGCTCATAAGC | 58.07 | 58.79 | 248 | 71 |
100.00%
|
61.54%
|
| Primer 176 | Clostridioides | TGTGATGAACCTGTTTCTGCA | TCTTGTATTGGTACTGCGCTC | 58.07 | 58.11 | 254 | 71 |
100.00%
|
61.54%
|
| Primer 177 | Clostridioides | GCGGTTATGCCATGCTACC | AACCAAAGAAGCCAGCAGTCT | 59.05 | 60.13 | 168 | 71 |
100.00%
|
61.54%
|
| Primer 178 | Clostridioides | ACTGTTGAGGCTCATTGGC | TCCGCTTCCACATGGGCAT | 58.05 | 61.98 | 156 | 71 |
100.00%
|
61.54%
|
| Primer 179 | Clostridioides | ACTGTTGAGGCTCATTGGC | GCTTCCACATGGGCATGAATC | 58.05 | 59.93 | 153 | 71 |
100.00%
|
69.23%
|
| Primer 180 | Clostridioides | TGTATGGTTCGGGTGTTGTG | TCCAAACTCCAGTACACCACT | 58.11 | 58.60 | 216 | 71 |
100.00%
|
69.23%
|