| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 201 | Clostridioides | ACCAGCAATGAGAGAGCTAGA | ATAGCACTCCCCACACCAGT | 58.26 | 60.55 | 237 | 69 |
100.00%
|
61.54%
|
| Primer 202 | Clostridioides | ACCAGCAATGAGAGAGCTAGA | TATAGCACTCCCCACACCAGT | 58.26 | 60.27 | 238 | 69 |
100.00%
|
61.54%
|
| Primer 203 | Clostridioides | GCGGTTATGCCATGCTACC | ACCAAAGAAGCCAGCAGTCTT | 59.05 | 60.13 | 167 | 69 |
100.00%
|
61.54%
|
| Primer 204 | Clostridioides | TGGCACTCTGGATGGGGAT | TGCACCATTTCCAGCACC | 60.31 | 58.22 | 179 | 69 |
100.00%
|
61.54%
|
| Primer 205 | Clostridioides | TGGCACTCTGGATGGGGAT | TTGCACCATTTCCAGCACC | 60.31 | 58.95 | 180 | 69 |
100.00%
|
61.54%
|
| Primer 206 | Clostridioides | GCAGTTACAACACCTGCCC | TGTGCCTTATCTGCTCTCCC | 59.04 | 59.17 | 269 | 68 |
100.00%
|
69.23%
|
| Primer 207 | Clostridioides | GCAGTTACAACACCTGCCC | TTGTGCCTTATCTGCTCTCCC | 59.04 | 59.79 | 270 | 68 |
100.00%
|
69.23%
|
| Primer 208 | Clostridioides | GCAGTTACAACACCTGCCC | ACCTCTAGCTTGTGCAACTGT | 59.04 | 59.58 | 165 | 68 |
100.00%
|
69.23%
|
| Primer 209 | Clostridioides | GCAGTTACAACACCTGCCC | GTGCCTTATCTGCTCTCCCAT | 59.04 | 59.58 | 268 | 68 |
100.00%
|
69.23%
|
| Primer 210 | Clostridioides | ACCTACAGCTGGTTTAGACCC | CCATGGGGATTCTAGACCAGC | 59.09 | 59.93 | 232 | 68 |
100.00%
|
69.23%
|