| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 261 | Clostridioides | ACCAGCAATGAGAGAGCTAGA | CACTCCCCACACCAGTTCTA | 58.26 | 58.65 | 233 | 66 |
100.00%
|
61.54%
|
| Primer 262 | Clostridioides | ACCTAGAAGTGGGCTTGCC | CAGTTGAGCCAAACCCACC | 59.31 | 58.96 | 242 | 66 |
100.00%
|
61.54%
|
| Primer 263 | Clostridioides | ACCTAGAAGTGGGCTTGCC | CCAGTTGAGCCAAACCCAC | 59.31 | 58.96 | 243 | 66 |
100.00%
|
61.54%
|
| Primer 264 | Clostridioides | ATTTGCAGGGCTTCGAAGTT | TCAGCACCACTTCTCACACC | 58.38 | 59.89 | 221 | 66 |
100.00%
|
69.23%
|
| Primer 265 | Clostridioides | GACTAGAGGTGTGCCAATTGC | TGCCCTCTGTCCACTAGCA | 59.26 | 60.23 | 294 | 66 |
100.00%
|
61.54%
|
| Primer 266 | Clostridioides | ACTAGAGGTGTGCCAATTGC | TGCCCTCTGTCCACTAGCA | 58.17 | 60.23 | 293 | 66 |
100.00%
|
61.54%
|
| Primer 267 | Clostridioides | GACTAGAGGTGTGCCAATTGC | ATGCCCTCTGTCCACTAGCA | 59.26 | 60.62 | 295 | 66 |
100.00%
|
61.54%
|
| Primer 268 | Clostridioides | ACTAGAGGTGTGCCAATTGC | ATGCCCTCTGTCCACTAGCA | 58.17 | 60.62 | 294 | 66 |
100.00%
|
61.54%
|
| Primer 269 | Clostridioides | GCGGAAGGTCGATTTATTGCA | ACCAAAGGCATGCTGAACTC | 59.33 | 58.75 | 269 | 66 |
100.00%
|
61.54%
|
| Primer 270 | Clostridioides | ATGGAGAGGAAGGGAGCTCC | ACTCCCATAGGCGAATCCTCT | 60.40 | 60.13 | 278 | 66 |
100.00%
|
61.54%
|