| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 311 | Clostridioides | TGGAGCACGCGATTATGAAGA | TGTCAACCCCTGCAGTTCT | 59.86 | 58.77 | 230 | 64 |
100.00%
|
61.54%
|
| Primer 312 | Clostridioides | GGTGCTGGTGCTGTTGTTG | AACCAGCAACAACGGCAC | 59.93 | 59.20 | 187 | 64 |
100.00%
|
69.23%
|
| Primer 313 | Clostridioides | ACGAAGGAGAAGAATGTGGCT | GGTGGAGTCATTGCCCAGA | 59.37 | 59.31 | 172 | 64 |
100.00%
|
61.54%
|
| Primer 314 | Clostridioides | AGCAGCTGGGGTTGGTAT | GCTCTGCCCATTCTCCACT | 58.16 | 59.39 | 284 | 63 |
100.00%
|
69.23%
|
| Primer 315 | Clostridioides | GCAGTTACAACACCTGCCC | GTGCCTTATCTGCTCTCCCA | 59.04 | 59.17 | 268 | 63 |
100.00%
|
69.23%
|
| Primer 316 | Clostridioides | AGCGGTACTTCTCAAAGACCA | AGCTGCTTCCTTGTTTCCTGT | 59.03 | 60.13 | 162 | 63 |
100.00%
|
69.23%
|
| Primer 317 | Clostridioides | AGCGGTACTTCTCAAAGACCA | GCTGCTTCCTTGTTTCCTGT | 59.03 | 58.68 | 161 | 63 |
100.00%
|
69.23%
|
| Primer 318 | Clostridioides | AGCGGTACTTCTCAAAGACCA | AGCTGCTTCCTTGTTTCCTG | 59.03 | 58.39 | 162 | 63 |
100.00%
|
69.23%
|
| Primer 319 | Clostridioides | AGCGGTACTTCTCAAAGACCA | TAGCTGCTTCCTTGTTTCCTG | 59.03 | 58.22 | 163 | 63 |
100.00%
|
69.23%
|
| Primer 320 | Clostridioides | CCTACAGCTGGTTTAGACCCA | CCATGGGGATTCTAGACCAGC | 59.09 | 59.93 | 231 | 63 |
100.00%
|
69.23%
|