| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 441 | Clostridioides | GCGGTACTTCTCAAAGACCAA | GCTGCTTCCTTGTTTCCTGT | 58.24 | 58.68 | 160 | 58 |
100.00%
|
69.23%
|
| Primer 442 | Clostridioides | GCGGTACTTCTCAAAGACCAA | AGCTGCTTCCTTGTTTCCTG | 58.24 | 58.39 | 161 | 58 |
100.00%
|
69.23%
|
| Primer 443 | Clostridioides | GCGGTACTTCTCAAAGACCAA | GCTGCTTCCTTGTTTCCTGTA | 58.24 | 58.50 | 160 | 58 |
100.00%
|
69.23%
|
| Primer 444 | Clostridioides | GCGGTACTTCTCAAAGACCAA | TAGCTGCTTCCTTGTTTCCTG | 58.24 | 58.22 | 162 | 58 |
100.00%
|
69.23%
|
| Primer 445 | Clostridioides | TGGAGCACGCGATTATGAAGA | CCCCTGCAGTTCTTGAAGCT | 59.86 | 60.25 | 224 | 58 |
100.00%
|
69.23%
|
| Primer 446 | Clostridioides | TGGAGCACGCGATTATGAAGA | CCCCTGCAGTTCTTGAAGC | 59.86 | 58.74 | 224 | 58 |
100.00%
|
69.23%
|
| Primer 447 | Clostridioides | TGGAGCACGCGATTATGAAGA | ACCCCTGCAGTTCTTGAAGC | 59.86 | 60.54 | 225 | 58 |
100.00%
|
69.23%
|
| Primer 448 | Clostridioides | TGGAGCACGCGATTATGAAGA | TGTCAACCCCTGCAGTTCTT | 59.86 | 59.45 | 230 | 58 |
100.00%
|
61.54%
|
| Primer 449 | Clostridioides | TGGCAGCTCCATCAACTGT | ACCAAGCCCTGCTCCCATA | 59.24 | 60.62 | 188 | 58 |
100.00%
|
61.54%
|
| Primer 450 | Clostridioides | ACCTCTTGCACATTCAACCAA | CCGCTTCGTTTGTCCAAGC | 58.33 | 60.08 | 229 | 58 |
100.00%
|
53.85%
|