| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 511 | Clostridioides | TTGCAGTAGGTGGTCTTGCTA | TGCCCATGCAGCAGTTGT | 59.03 | 60.20 | 182 | 55 |
100.00%
|
69.23%
|
| Primer 512 | Clostridioides | GGGCTTGCTTGTCCTAAACC | CGTCATTTCCAAGTCCCATCT | 59.11 | 58.01 | 295 | 55 |
100.00%
|
61.54%
|
| Primer 513 | Clostridioides | ATGTATGGTTCGGGTGTTGTG | TCCAAACTCCAGTACACCACT | 58.57 | 58.60 | 217 | 55 |
100.00%
|
69.23%
|
| Primer 514 | Clostridioides | AGGAGGAAGACTTACAGGGC | ACGCCTCTCATTGTCATGCA | 58.43 | 60.04 | 180 | 55 |
100.00%
|
61.54%
|
| Primer 515 | Clostridioides | AGCAGCAGCAGGATGTAACT | GCACTCTTTCTCCATCCCCAG | 59.38 | 60.41 | 237 | 55 |
100.00%
|
61.54%
|
| Primer 516 | Clostridioides | TGTTGATGCCGACCCTGA | CCACCAGAATCTACGGTTCCC | 58.52 | 60.13 | 240 | 54 |
100.00%
|
61.54%
|
| Primer 517 | Clostridioides | AAGGCGTAGAACGTGTGCA | ATCACGCCTGCTGCAACA | 59.93 | 60.28 | 163 | 54 |
100.00%
|
38.46%
|
| Primer 518 | Clostridioides | CTGCAAAGGCTGGAGCAAA | CACTATGCACCCCATGTTGC | 58.96 | 59.54 | 192 | 54 |
100.00%
|
61.54%
|
| Primer 519 | Clostridioides | ACGTTGTGCAGGTGGAGT | TCGCCAGCCACATCTATCA | 59.09 | 58.48 | 180 | 54 |
100.00%
|
69.23%
|
| Primer 520 | Clostridioides | AGACGTTGTGCAGGTGGAG | TCGCCAGCCACATCTATCA | 59.93 | 58.48 | 182 | 54 |
100.00%
|
69.23%
|