| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 751 | Clostridioides | GCCGAAAGAGATGGCGAAG | TGAGCCAGCATCAGCAGA | 58.99 | 58.60 | 174 | 42 |
100.00%
|
69.23%
|
| Primer 752 | Clostridioides | ACATGAGTTTGGCCATGCTT | AAAACGCCTGCAAGTGAAAC | 58.36 | 58.08 | 219 | 42 |
100.00%
|
69.23%
|
| Primer 753 | Clostridioides | CATGACTGCGCAAGGCTT | TGGCTCTTGCAAGCTTTACAG | 58.73 | 59.12 | 247 | 42 |
100.00%
|
61.54%
|
| Primer 754 | Clostridioides | AGGCAAGAAGACATCCACAGA | ACCAGAAACATCATTGGCCAT | 59.02 | 58.18 | 239 | 42 |
100.00%
|
69.23%
|
| Primer 755 | Clostridioides | TCCACAAAGCCCTCAAGCC | GCCATAGCTATCCATGCCCTT | 60.23 | 59.99 | 213 | 42 |
100.00%
|
53.85%
|
| Primer 756 | Clostridioides | TGCCCTCAAGAAACTCACGA | TGCTGGGTCCATTTCTGCT | 59.24 | 59.23 | 201 | 42 |
100.00%
|
69.23%
|
| Primer 757 | Clostridioides | TTGCCCTCAAGAAACTCACGA | TGCTGGGTCCATTTCTGCT | 59.86 | 59.23 | 202 | 42 |
100.00%
|
69.23%
|
| Primer 758 | Clostridioides | GGAGGAAGACTTACAGGGCTT | ACGCCTCTCATTGTCATGCA | 59.09 | 60.04 | 179 | 42 |
100.00%
|
61.54%
|
| Primer 759 | Clostridioides | AGACCCCTCAGAAGGTGAAC | TCCTGATGCTCCTGCTTCTT | 58.65 | 58.72 | 172 | 42 |
100.00%
|
61.54%
|
| Primer 760 | Clostridioides | GGCTTACAGGTTGGGCACA | TCTAGCTCCGTCTCCTGTCAA | 60.23 | 60.00 | 158 | 41 |
100.00%
|
69.23%
|