| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium | AAAGTGCAACACCAGGCG | AGCTCCCTTGCAACTGGTC | 58.88 | 59.93 | 187 | 91 |
100.00%
|
48.28%
|
| Primer 2 | Clostridium | AAAGTGCAACACCAGGCG | AGCTCCCTTGCAACTGGTC | 58.88 | 59.93 | 187 | 91 |
100.00%
|
48.28%
|
| Primer 3 | Clostridium | AAAGTGCAACACCAGGCG | CTCCCTTGCAACTGGTCCA | 58.88 | 59.54 | 185 | 91 |
100.00%
|
41.38%
|
| Primer 4 | Clostridium | TTCGGAGTAAGCGCTGCTG | TGTCTTAGGAGCTCCGTCAAC | 60.45 | 59.46 | 198 | 81 |
95.00%
|
32.76%
|
| Primer 5 | Clostridium | AAAGTGCAACACCAGGCG | TCCCTTGCAACTGGTCCAA | 58.88 | 59.07 | 184 | 80 |
100.00%
|
41.38%
|
| Primer 6 | Clostridium | AAAGTGCAACACCAGGCG | CTCCCTTGCAACTGGTCCAA | 58.88 | 60.18 | 185 | 80 |
100.00%
|
41.38%
|
| Primer 7 | Clostridium | AAAGTGCAACACCAGGCG | TCTCTTGCAACTGGTCCGAA | 58.88 | 59.24 | 184 | 79 |
95.45%
|
36.21%
|
| Primer 8 | Clostridium | AAAGTGCAACACCAGGCG | CTCTCTTGCAACTGGTCCG | 58.88 | 58.17 | 185 | 79 |
95.45%
|
36.21%
|
| Primer 9 | Clostridium | AAAGTGCAACACCAGGCG | CTCTCTTGCAACTGGTCCGA | 58.88 | 59.68 | 185 | 73 |
95.45%
|
36.21%
|
| Primer 10 | Clostridium | AAAGTGCAACACCAGGCG | TCTCTTGCAACTGGTCCGA | 58.88 | 58.56 | 184 | 73 |
95.45%
|
36.21%
|