| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Collinsella | CTACGTTTGCGCTCATGGC | TCGTTCGCACATCGCTGT | 59.94 | 60.05 | 231 | 143 |
100.00%
|
64.91%
|
| Primer 2 | Collinsella | TACGTTTGCGCTCATGGC | TCGTTCGCACATCGCTGT | 58.83 | 60.05 | 230 | 143 |
100.00%
|
64.91%
|
| Primer 3 | Collinsella | ATTTGGCGTCGTCGGTGT | ATGCCTTCCTTGTGCGCA | 59.97 | 60.28 | 171 | 138 |
100.00%
|
59.65%
|
| Primer 4 | Collinsella | AGTACGCCTGTGACGTGAC | ATGAGCTCGATGGCAGCA | 59.71 | 59.09 | 160 | 138 |
100.00%
|
68.42%
|
| Primer 6 | Collinsella | TCGTTTGGGCTGTCTCTGC | TGCACAGGACGACACACA | 60.30 | 59.10 | 192 | 137 |
100.00%
|
36.84%
|
| Primer 7 | Collinsella | TGGCAACACGCGTCTGT | GCCCAAAAGCTTGCGCAT | 59.85 | 60.05 | 274 | 137 |
100.00%
|
12.28%
|
| Primer 9 | Collinsella | TCGTTTGGGCTGTCTCTGC | TGCACAGGACGACACACAC | 60.30 | 60.52 | 192 | 135 |
100.00%
|
70.18%
|
| Primer 10 | Collinsella | TGGCAACACGCGTCTGTA | GCCCAAAAGCTTGCGCAT | 59.58 | 60.05 | 274 | 133 |
100.00%
|
12.28%
|
| Primer 11 | Collinsella | TGATGAAGCAGGCTCGCA | TGTTGGCGAGCTCGTTGA | 59.34 | 59.58 | 243 | 132 |
100.00%
|
68.42%
|
| Primer 12 | Collinsella | TGCTCAACGACCGCATCA | TGTCCAGAATCGCCAAGCC | 59.66 | 60.38 | 168 | 132 |
100.00%
|
70.18%
|