| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Comamonas | ATGCACGCATGGCTTTGC | AAGACAGGCCACATGCTGG | 60.13 | 60.30 | 264 | 157 |
100.00%
|
33.33%
|
| Primer 2 | Comamonas | GTGCGCTTTGACTGGGTTG | CGCGTTGCCACAATGCAT | 60.01 | 60.13 | 223 | 149 |
100.00%
|
33.33%
|
| Primer 3 | Comamonas | TGGAATCTCTGCGTGCCTG | TGCTGATGATGGCCACCAG | 60.08 | 60.08 | 255 | 148 |
100.00%
|
33.33%
|
| Primer 4 | Comamonas | TCTCTGGTCATGCTGCTGG | AGCAGGAACTTGGCCATGG | 59.40 | 60.30 | 224 | 145 |
100.00%
|
33.33%
|
| Primer 5 | Comamonas | GCGCTTTGACTGGGTTGAC | CGCGTTGCCACAATGCAT | 59.72 | 60.13 | 221 | 141 |
100.00%
|
33.33%
|
| Primer 6 | Comamonas | ACAAGTGCCTGACGCTGT | ATGTGCTTGCGCTGTGGA | 59.49 | 60.28 | 286 | 140 |
100.00%
|
33.33%
|
| Primer 7 | Comamonas | ACGAAGATGGCAACGGCA | TGCATCCATCACGCCTTGT | 59.97 | 60.00 | 233 | 138 |
100.00%
|
33.33%
|
| Primer 8 | Comamonas | ACGAAGATGGCAACGGCA | CCAAAATCTGCGGTGCCTG | 59.97 | 59.79 | 298 | 138 |
100.00%
|
33.33%
|
| Primer 9 | Comamonas | ACGAAGATGGCAACGGCA | AATCTGCGGTGCCTGAGAG | 59.97 | 59.78 | 294 | 138 |
100.00%
|
33.33%
|
| Primer 10 | Comamonas | ACGAAGATGGCAACGGCA | ACCAAAATCTGCGGTGCC | 59.97 | 58.63 | 299 | 138 |
100.00%
|
33.33%
|