| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Cronobacter | ACTGACTGAACTGGCGAGC | GCGTTATCGTCGCGAATGG | 60.01 | 59.80 | 177 | 165 |
100.00%
|
75.00%
|
| Primer 2 | Cronobacter | TTAAACGTCGCCTCCCTGG | AACCGTCGTCGTCATCCTG | 59.71 | 59.79 | 235 | 147 |
100.00%
|
50.00%
|
| Primer 3 | Cronobacter | TGCTTTTCGGCATGCTGC | AGCGTGGCGATTTTCTGC | 59.74 | 59.13 | 217 | 146 |
100.00%
|
75.00%
|
| Primer 4 | Cronobacter | TGCTTTTCGGCATGCTGC | AGCAGCGTGGCGATTTTC | 59.74 | 59.13 | 220 | 146 |
100.00%
|
75.00%
|
| Primer 5 | Cronobacter | GCCAAAACGCCTGTTGCA | TGTTTACCGTGGCCATGCA | 59.89 | 60.23 | 279 | 138 |
100.00%
|
75.00%
|
| Primer 6 | Cronobacter | GCCAAAACGCCTGTTGCA | TACCGTGGCCATGCATCAC | 59.89 | 60.45 | 275 | 138 |
100.00%
|
75.00%
|
| Primer 7 | Cronobacter | AGTATCGCGACGCTTGCA | CGTGATCGAGGCTTGCCAT | 59.82 | 60.52 | 220 | 138 |
100.00%
|
50.00%
|
| Primer 8 | Cronobacter | ATCGCGACGGATTACCACC | GCTTTCCAGAAACGCCACC | 59.93 | 59.71 | 249 | 138 |
100.00%
|
75.00%
|
| Primer 9 | Cronobacter | TACCGCTTACGATCGCACC | TCGTCCAGCGCAACGAAA | 59.94 | 60.28 | 204 | 137 |
100.00%
|
50.00%
|
| Primer 10 | Cronobacter | GCCATTGGCCTGTGTTGTC | TGCGCACCAGGTAAACCA | 59.71 | 59.49 | 292 | 135 |
100.00%
|
50.00%
|