| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Cupriavidus | TGCTCGACATGAAGCGCT | GTCGGCTGCAAGGTTGAAC | 59.74 | 59.72 | 293 | 50 |
100.00%
|
66.67%
|
| Primer 62 | Cupriavidus | TGCTGTACGTGCTGCTGT | TGACCATCATGCGCAGGT | 59.58 | 59.33 | 162 | 50 |
100.00%
|
66.67%
|
| Primer 63 | Cupriavidus | TGCTGTACGTGCTGCTGT | TTGACCATCATGCGCAGGT | 59.58 | 60.00 | 163 | 50 |
100.00%
|
66.67%
|
| Primer 64 | Cupriavidus | GCAAGGACGGCTCGATCAT | TGGACTGCTTGTAGCGCT | 60.23 | 58.94 | 225 | 50 |
100.00%
|
66.67%
|
| Primer 65 | Cupriavidus | ACGCACGCAAGACCATCA | TGATGATCAGCGCACGGTT | 59.97 | 60.08 | 243 | 49 |
100.00%
|
100.00%
|
| Primer 66 | Cupriavidus | AGGAAACGCTCGAACGCA | AGTCGCGTTGCCATTCCA | 59.97 | 59.97 | 291 | 49 |
100.00%
|
66.67%
|
| Primer 67 | Cupriavidus | TCCAGCGCTCGGTCAATTT | TGTACGGCACATGCAGCA | 60.00 | 59.97 | 246 | 49 |
100.00%
|
66.67%
|
| Primer 68 | Cupriavidus | ACGTCATGTCGCTGCTGT | AACGCGTTCATGTTGCCG | 59.66 | 59.75 | 193 | 49 |
100.00%
|
66.67%
|
| Primer 69 | Cupriavidus | TGCTGTACGTGCTGCTGT | AACGCGTTCATGTTGCCG | 59.58 | 59.75 | 181 | 49 |
100.00%
|
66.67%
|
| Primer 70 | Cupriavidus | TGCCTTCATCGCCACCAA | AACGCGTTCATGTTGCCG | 59.57 | 59.75 | 294 | 49 |
100.00%
|
66.67%
|