| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Dehalococcoides | ACTTGACCAGTCTGCGCT | TCTATGCTTACGCCAGCCG | 58.86 | 59.93 | 246 | 84 |
100.00%
|
25.00%
|
| Primer 102 | Dehalococcoides | TGAAACACACTGCCCTGCT | TGCCGTTTTCCAGCAGGT | 59.77 | 59.81 | 237 | 84 |
100.00%
|
50.00%
|
| Primer 103 | Dehalococcoides | TTTCATGGCACCGCTGCT | CGCCAAGCCAAAAGCCAA | 60.28 | 59.58 | 193 | 84 |
100.00%
|
25.00%
|
| Primer 104 | Dehalococcoides | TTTCATGGCACCGCTGCT | AACGCCAAGCCAAAAGCC | 60.28 | 59.58 | 195 | 84 |
100.00%
|
25.00%
|
| Primer 105 | Dehalococcoides | TGTGTTTGCGCTGCGAAG | AGCAGGCTTTGGGGTTTGT | 59.67 | 60.08 | 282 | 84 |
100.00%
|
50.00%
|
| Primer 106 | Dehalococcoides | AGACGGAAGCAGCATGGAC | GGTGGGGAAAGCTTTGGGA | 60.08 | 59.84 | 262 | 84 |
100.00%
|
25.00%
|
| Primer 107 | Dehalococcoides | AGCCAAAGTGCTGGTGGT | TGCTGCCGTGTTTGGTCA | 59.40 | 60.12 | 242 | 84 |
100.00%
|
50.00%
|
| Primer 108 | Dehalococcoides | TGAAGTGGCAGGCATAGGC | ACTGCTGCCGTGTTTGGT | 60.08 | 60.12 | 160 | 84 |
100.00%
|
100.00%
|
| Primer 109 | Dehalococcoides | ACGGCAAGGGCAACTTCT | TCTTGCCCACGTGCTCAA | 59.48 | 59.49 | 225 | 83 |
100.00%
|
25.00%
|
| Primer 110 | Dehalococcoides | ATTGCTCAAGCCAGGGCA | TGAGCCGACAAACTGCCAT | 59.56 | 59.93 | 290 | 82 |
100.00%
|
100.00%
|