| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 181 | Dehalococcoides | TTCAAACGGCTGCGCAAC | CAGCTCCAGCCGTTCCTTA | 59.97 | 59.40 | 177 | 67 |
100.00%
|
50.00%
|
| Primer 182 | Dehalococcoides | CGCACCAAACGGGAAAAGG | AACCGTGTGCCAAAGCCT | 60.01 | 60.12 | 166 | 66 |
100.00%
|
25.00%
|
| Primer 183 | Dehalococcoides | TGCGCAACAAGGTAGCCT | TGCCGATTTGCCACACCT | 59.57 | 59.88 | 283 | 66 |
100.00%
|
100.00%
|
| Primer 184 | Dehalococcoides | TGCGCAACAAGGTAGCCT | ATTTGCCACACCTGCGGT | 59.57 | 60.20 | 278 | 66 |
100.00%
|
100.00%
|
| Primer 185 | Dehalococcoides | TGCGCAACAAGGTAGCCT | TTTGCCACACCTGCGGTA | 59.57 | 59.49 | 277 | 66 |
100.00%
|
100.00%
|
| Primer 186 | Dehalococcoides | TGCGCAACAAGGTAGCCT | TGCTGCCGATTTGCCACA | 59.57 | 60.60 | 286 | 66 |
100.00%
|
100.00%
|
| Primer 187 | Dehalococcoides | TGATTGCGGTGGCCAGTT | TTCCATGGCACCAGCGTT | 59.88 | 59.88 | 169 | 66 |
100.00%
|
50.00%
|
| Primer 188 | Dehalococcoides | GCCGTTGAGAAAATGCGCA | ACCAGTTCGCTTTCGCTGA | 60.08 | 59.93 | 173 | 65 |
100.00%
|
100.00%
|
| Primer 189 | Dehalococcoides | AAAACCAGCTGCAGCCGA | CCGCTGCGCAAAAGTGAT | 60.20 | 59.44 | 247 | 65 |
100.00%
|
25.00%
|
| Primer 190 | Dehalococcoides | AAAACCAGCTGCAGCCGA | GGCCATCACGTCTTTGGGA | 60.20 | 60.00 | 212 | 65 |
100.00%
|
25.00%
|