| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Dehalococcoides | TTGGCAGTTACTCCCAGCG | GGCAGTGCGCAGTTTCAA | 60.00 | 59.28 | 269 | 57 |
100.00%
|
75.00%
|
| Primer 222 | Dehalococcoides | TTTGCTGACACTCCCTCCG | TGTCATTCAGGCTGGCGT | 59.63 | 59.25 | 247 | 56 |
100.00%
|
50.00%
|
| Primer 223 | Dehalococcoides | ACCGCTTTGGCAGTCCAT | CGGTGAGGCTGTTTTCCCT | 59.56 | 59.93 | 299 | 55 |
100.00%
|
50.00%
|
| Primer 224 | Dehalococcoides | ATCCTGACGGCATAGGCAG | GGAACAATGGCTGCGGTT | 59.25 | 58.63 | 248 | 55 |
100.00%
|
25.00%
|
| Primer 225 | Dehalococcoides | CCGCTTTGCCGAACAGTT | TGTCAGCTGGCAGTGCTT | 58.97 | 59.49 | 172 | 55 |
100.00%
|
25.00%
|
| Primer 226 | Dehalococcoides | TTGCCAGCTACGCCATGA | ACCTTCCAGCACCGCAAA | 59.33 | 59.81 | 254 | 54 |
100.00%
|
50.00%
|
| Primer 227 | Dehalococcoides | GCGCAAAGAACTGGTACGC | AGCCAAATGGTCACGCGA | 60.15 | 59.97 | 157 | 53 |
100.00%
|
25.00%
|
| Primer 228 | Dehalococcoides | GCCTGAACTGCGCAAAGA | AGCCAAATGGTCACGCGA | 58.66 | 59.97 | 166 | 53 |
100.00%
|
25.00%
|
| Primer 229 | Dehalococcoides | ACGTGAAGAAGAGCAGCGT | AGCCAAATGGTCACGCGA | 59.64 | 59.97 | 256 | 53 |
100.00%
|
25.00%
|
| Primer 230 | Dehalococcoides | AAGAAGAGCAGCGTCAGGC | AGCCAAATGGTCACGCGA | 60.38 | 59.97 | 251 | 53 |
100.00%
|
25.00%
|