| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Dehalococcoides | AGCCCAAACGTCAGCTGA | TTTGCAAAGCAGCTCGCC | 59.17 | 59.66 | 227 | 110 |
100.00%
|
100.00%
|
| Primer 32 | Dehalococcoides | TGCTTTGTTTGCGGTGGC | ACCCCACCAGCATAAGCAC | 59.89 | 60.00 | 221 | 110 |
100.00%
|
50.00%
|
| Primer 33 | Dehalococcoides | AACAGGCTCACGCTCCAA | TAAACCGCAGAGCACGCA | 59.17 | 59.97 | 226 | 108 |
100.00%
|
25.00%
|
| Primer 34 | Dehalococcoides | GCCAGCTGGTTGAAGTGGA | TGTACAGCTGGCGATGTCC | 60.23 | 59.78 | 231 | 107 |
100.00%
|
75.00%
|
| Primer 35 | Dehalococcoides | CGCCTCAGCTACCCCAAAA | TGGCATCACGCATCTGCT | 60.00 | 59.73 | 202 | 106 |
100.00%
|
50.00%
|
| Primer 36 | Dehalococcoides | ATTGGCGAAGGTGCAGCT | TGCATCATCAGGGCTGCA | 59.97 | 59.32 | 263 | 106 |
100.00%
|
50.00%
|
| Primer 37 | Dehalococcoides | AGGCTTATGCGGTGCTGAA | TGCCCTTTCGCACAAACG | 59.70 | 59.28 | 185 | 106 |
100.00%
|
100.00%
|
| Primer 38 | Dehalococcoides | AGCCCAAACGTCAGCTGAA | TTTGCAAAGCAGCTCGCC | 59.85 | 59.66 | 227 | 106 |
100.00%
|
100.00%
|
| Primer 39 | Dehalococcoides | ATCCTGACGGCATAGGCAG | GGAACAATGGCTGCGGTTC | 59.25 | 59.79 | 248 | 106 |
100.00%
|
75.00%
|
| Primer 40 | Dehalococcoides | CGAGCTGGAAAAGGCAGGT | AAGGCTTTCAGCTGGGCA | 60.30 | 59.48 | 213 | 106 |
100.00%
|
25.00%
|