| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 511 | Desulfitobacterium | GCCGTTCTGCCAGAAAAGC | TGGGGTGTAGCAATCACGG | 60.08 | 59.70 | 164 | 68 |
100.00%
|
100.00%
|
| Primer 512 | Desulfitobacterium | ATGCTGCCTGCCACGATT | GCCAGACGGGACAATGGAT | 60.04 | 59.78 | 203 | 68 |
100.00%
|
100.00%
|
| Primer 513 | Desulfitobacterium | ATGCTGCCTGCCACGATT | GCTTCCAGTCTCTTGGCCA | 60.04 | 59.62 | 218 | 68 |
100.00%
|
100.00%
|
| Primer 514 | Desulfitobacterium | TCCCGTGATTCGTGCCAA | TTCGCTGCCTTCTCCCATG | 59.26 | 60.08 | 271 | 68 |
100.00%
|
100.00%
|
| Primer 515 | Desulfitobacterium | TGCCAATCCTGCCGATGT | AGCACCTTCATCGCCACA | 59.32 | 59.25 | 255 | 68 |
100.00%
|
50.00%
|
| Primer 516 | Desulfitobacterium | TGCCAATCCTGCCGATGT | AAGCACCTTCATCGCCACA | 59.32 | 59.93 | 256 | 68 |
100.00%
|
100.00%
|
| Primer 517 | Desulfitobacterium | ATACGAACGCCTGGGCAT | TCCCACACTTGCCACATCC | 59.09 | 59.93 | 175 | 68 |
100.00%
|
100.00%
|
| Primer 518 | Desulfitobacterium | GTCTGGTTTCGGTGCTGGA | TGGGGTCTGGCAATCACA | 59.93 | 58.41 | 205 | 68 |
100.00%
|
100.00%
|
| Primer 519 | Desulfitobacterium | TGGTCTGGTTTCGGTGCTG | TGGGGTCTGGCAATCACA | 60.23 | 58.41 | 207 | 68 |
100.00%
|
100.00%
|
| Primer 520 | Desulfitobacterium | TCTGCACAGTGAACGCCA | AGGCAAGCATCAATGGCG | 59.50 | 58.80 | 234 | 68 |
100.00%
|
100.00%
|