| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Desulfurispirillum | CCCCACGCGTGCTATATGT | AAGCCACACTGTGCGTCA | 59.93 | 59.81 | 217 | 132 |
100.00%
|
100.00%
|
| Primer 2 | Desulfurispirillum | CCCGACGCATGACCTTTGA | AAGCCACACTGTGCGTCA | 60.38 | 59.81 | 252 | 132 |
100.00%
|
100.00%
|
| Primer 3 | Desulfurispirillum | ACATTCCCACTGACGCCAG | TGTGAATGCTGCGCAGGA | 60.00 | 59.97 | 177 | 129 |
100.00%
|
100.00%
|
| Primer 4 | Desulfurispirillum | ACATTCCCACTGACGCCA | TGTGAATGCTGCGCAGGA | 58.84 | 59.97 | 177 | 124 |
100.00%
|
100.00%
|
| Primer 5 | Desulfurispirillum | TTCACGAAGCCAGTGCCT | TCGATTTTGCGCTTGCCC | 59.17 | 59.43 | 269 | 122 |
100.00%
|
100.00%
|
| Primer 6 | Desulfurispirillum | AGCCGTGACAACCGCTTT | AGCGCACGACCTTTCTGA | 60.20 | 59.27 | 278 | 120 |
100.00%
|
100.00%
|
| Primer 7 | Desulfurispirillum | GTCACGGAACTGCTGGTCA | GCGATCATCCAGGGCAGAA | 59.93 | 59.85 | 197 | 117 |
100.00%
|
100.00%
|
| Primer 8 | Desulfurispirillum | TGTCACGGAACTGCTGGTC | GCGATCATCCAGGGCAGAA | 59.93 | 59.85 | 198 | 117 |
100.00%
|
100.00%
|
| Primer 9 | Desulfurispirillum | ACGGAAATCGGCACCATGT | TGCGCAACCGTAACTTGC | 60.00 | 59.37 | 226 | 115 |
100.00%
|
100.00%
|
| Primer 10 | Desulfurispirillum | GCCAGTGCCTATCGAACCA | TCGATTTTGCGCTTGCCC | 59.78 | 59.43 | 261 | 113 |
100.00%
|
100.00%
|