| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Dorea | AGAACTTCCGGACCGTCTG | GCACGTTCTGCAAACCTGG | 59.03 | 60.01 | 165 | 141 |
100.00%
|
20.00%
|
| Primer 22 | Dorea | AGAAGGTGCGGTGCTGAT | TGCACTTGCGCTTCCTGT | 58.93 | 60.20 | 184 | 141 |
100.00%
|
20.00%
|
| Primer 23 | Dorea | GAGAAGGTGCGGTGCTGAT | TGCACTTGCGCTTCCTGT | 60.08 | 60.20 | 185 | 141 |
100.00%
|
20.00%
|
| Primer 24 | Dorea | GCACACAAGCGCATGGAT | ACGTACCACCGCATCGATC | 59.12 | 59.93 | 240 | 141 |
100.00%
|
20.00%
|
| Primer 25 | Dorea | GCACACAAGCGCATGGAT | ACTTGTCCATACGGCTGGC | 59.12 | 60.08 | 154 | 141 |
100.00%
|
20.00%
|
| Primer 26 | Dorea | GCACACAAGCGCATGGAT | TTGTCCATACGGCTGGCAG | 59.12 | 60.08 | 152 | 141 |
100.00%
|
20.00%
|
| Primer 27 | Dorea | TTTCGAAGCGACAGGGGA | TGTCCGCATGCATTTGGC | 58.54 | 59.43 | 214 | 140 |
100.00%
|
40.00%
|
| Primer 28 | Dorea | CGGAGTCAGTGGAATCGGA | CCTGAACCGCTTCGCTTTG | 58.81 | 59.79 | 266 | 140 |
100.00%
|
20.00%
|
| Primer 29 | Dorea | TCGGAGTCAGTGGAATCGGA | CCTGAACCGCTTCGCTTTG | 60.32 | 59.79 | 267 | 140 |
100.00%
|
20.00%
|
| Primer 30 | Dorea | CCGGAAGATCTGGCGAAGT | TTGCTGCTGCCACATGGA | 59.48 | 59.88 | 247 | 140 |
100.00%
|
20.00%
|