| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1111 | Edwardsiella | TGAACGCCTGATGCTGCT | CGCCACGCTTGATGATGAC | 59.65 | 59.65 | 254 | 61 |
100.00%
|
20.00%
|
| Primer 1112 | Edwardsiella | TCACGCACGCTGTTGGAA | CGTGCCAACGCCTGAAAA | 60.20 | 59.28 | 152 | 61 |
100.00%
|
20.00%
|
| Primer 1113 | Edwardsiella | TCTGCGTTTGCCGTGGTA | GTGTTCGGCAGGATCAGCT | 59.58 | 60.08 | 227 | 61 |
100.00%
|
40.00%
|
| Primer 1114 | Edwardsiella | ACAGCTGCGTGAAGAGCT | AGCGAGGCCAAGAAAGCA | 59.26 | 59.57 | 210 | 61 |
100.00%
|
20.00%
|
| Primer 1115 | Edwardsiella | CGCGATGGAAGAGACCGAA | TGGCGTATCCTCACCTTGC | 59.86 | 59.78 | 181 | 61 |
100.00%
|
20.00%
|
| Primer 1116 | Edwardsiella | ACGGCAAAAGTCGTCGCA | CGATGATGGCCATGTCGGT | 60.59 | 60.23 | 253 | 61 |
100.00%
|
20.00%
|
| Primer 1117 | Edwardsiella | AATGTCCGATGCACCGCT | AGCGCTCTGTTGAAGACGG | 59.73 | 60.37 | 155 | 61 |
100.00%
|
40.00%
|
| Primer 1118 | Edwardsiella | AATGTCCGATGCACCGCT | GCAGCGCTCTGTTGAAGAC | 59.73 | 59.50 | 157 | 61 |
100.00%
|
40.00%
|
| Primer 1119 | Edwardsiella | TTTCAGCGCAGGGATGCT | ATGGCCAATGTCACCGCT | 59.65 | 59.64 | 187 | 61 |
100.00%
|
20.00%
|
| Primer 1120 | Edwardsiella | GCTGTTGCTGATGCGCAT | ATGGCCAATGTCACCGCT | 59.51 | 59.64 | 160 | 61 |
100.00%
|
20.00%
|