| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Edwardsiella | TATCTGGTGACGCTGGAGC | AGCGCAATGGCACCGATA | 59.18 | 59.81 | 236 | 119 |
100.00%
|
40.00%
|
| Primer 62 | Edwardsiella | TGCTGGCGTCACCACTATC | AACAGCAGCACCAGCAGT | 59.78 | 59.81 | 236 | 118 |
100.00%
|
20.00%
|
| Primer 63 | Edwardsiella | AATCCACCAGGTGTCGCTG | ACCTCGCGTACGCTAAAGG | 60.00 | 59.86 | 237 | 118 |
100.00%
|
20.00%
|
| Primer 64 | Edwardsiella | GTCGCGATTCCGAAAGCA | ACCGTCAGCCAAAGCGAA | 58.53 | 59.89 | 158 | 117 |
100.00%
|
40.00%
|
| Primer 65 | Edwardsiella | TCATGTCGCCAAACGGGT | TCCAGCTGTGACAGAGGCT | 59.57 | 60.53 | 204 | 117 |
100.00%
|
20.00%
|
| Primer 66 | Edwardsiella | TCATGTCGCCAAACGGGT | ATCCAGCTGTGACAGAGGC | 59.57 | 59.40 | 205 | 117 |
100.00%
|
20.00%
|
| Primer 67 | Edwardsiella | TCCATGAGTTCAGCTGCGG | TGTCCTGCATGGCATGGT | 60.08 | 59.23 | 288 | 117 |
100.00%
|
20.00%
|
| Primer 68 | Edwardsiella | TGAGTTCAGCTGCGGTGA | TGTCCTGCATGGCATGGT | 58.86 | 59.23 | 284 | 117 |
100.00%
|
20.00%
|
| Primer 69 | Edwardsiella | ACGCGAGCAGTTGACGAT | ACCGCTTTCATCGCCTCTC | 59.74 | 60.15 | 210 | 116 |
100.00%
|
20.00%
|
| Primer 70 | Edwardsiella | AGCAACAGCACGCCATCT | TGCCAGCCGTCTTTGATCA | 59.97 | 59.63 | 166 | 116 |
100.00%
|
40.00%
|