| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Enorma | TTGCCCAAGCGCTTTTGG | AAAACGCGTGTGCACAGG | 59.58 | 59.59 | 181 | 167 |
100.00%
|
50.00%
|
| Primer 2 | Enorma | TGTGCAACGCTGGGATGT | AGACAGCCTTGCCGTTCAT | 59.89 | 59.62 | 240 | 148 |
100.00%
|
50.00%
|
| Primer 3 | Enorma | CGATTCGTGCTTCTGCGTG | ACTTCTGGCATGCGCAGT | 59.94 | 59.97 | 296 | 143 |
100.00%
|
50.00%
|
| Primer 4 | Enorma | TCAGCAGGCCTCTCTAGCA | TGCGGTTGCTAAGCTGCA | 60.00 | 60.28 | 229 | 138 |
100.00%
|
50.00%
|
| Primer 5 | Enorma | TCCGGAAACCACTGGCTTC | TGCGGTTGCTAAGCTGCA | 59.93 | 60.28 | 293 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Enorma | ATGGCACGCCTGCAACT | TGCGGTTGCTAAGCTGCA | 59.93 | 60.28 | 264 | 138 |
100.00%
|
50.00%
|
| Primer 7 | Enorma | CAACAACTGGCACGTGCA | ATGGCGATGGCAATGTGC | 59.20 | 59.19 | 189 | 138 |
100.00%
|
50.00%
|
| Primer 8 | Enorma | TCCTTGCGGTGTCAAGCA | TGGCGAAGATGAGGAGCAC | 59.49 | 59.78 | 207 | 138 |
100.00%
|
50.00%
|
| Primer 9 | Enorma | TCCTTGCGGTGTCAAGCA | ACGGCAAGGAATAGCAGGC | 59.49 | 60.45 | 163 | 138 |
100.00%
|
50.00%
|
| Primer 10 | Enorma | TCCTTGCGGTGTCAAGCA | AAGATGAGGAGCACGAGGC | 59.49 | 59.48 | 202 | 138 |
100.00%
|
50.00%
|