| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Escherichia | TGCACGAGCGCTGAAGAA | TCTGCTTCAACGCCACCA | 59.97 | 59.49 | 293 | 117 |
100.00%
|
50.00%
|
| Primer 2 | Escherichia | TCTCGGTTTCACGCTGCA | AAACGCCAGATGACGCAG | 59.58 | 58.43 | 163 | 116 |
90.91%
|
166.67%
|
| Primer 3 | Escherichia | TCTCGGTTTCACGCTGCA | AAACGCCAGATGACGCAGG | 59.58 | 61.03 | 163 | 116 |
91.67%
|
183.33%
|
| Primer 4 | Escherichia | TGACACCATCGAAGCGCA | ATCGCCTGGAAACGCTGA | 59.66 | 59.34 | 222 | 112 |
91.67%
|
183.33%
|
| Primer 5 | Escherichia | TCAGCCGTGTTTTAGCCCT | GTGCATGTTGCCGTTGCT | 59.24 | 59.66 | 248 | 111 |
100.00%
|
50.00%
|
| Primer 6 | Escherichia | TCTCGGTTTCACGCTGCA | CGCTAAACGCCAGATGACG | 59.58 | 59.37 | 167 | 110 |
91.67%
|
183.33%
|
| Primer 7 | Escherichia | GCGTTCGCGTCCTCTTTT | TCGCTACGGGTTTTGAGCA | 58.76 | 59.63 | 158 | 109 |
100.00%
|
50.00%
|
| Primer 8 | Escherichia | GTTCAGCCGTGTTTTAGCCC | GTGCATGTTGCCGTTGCT | 59.76 | 59.66 | 250 | 106 |
100.00%
|
50.00%
|
| Primer 9 | Escherichia | TGCACGAGCGCTGAAGAA | GCTTCAACGCCACCAACTG | 59.97 | 60.01 | 290 | 106 |
100.00%
|
50.00%
|
| Primer 10 | Escherichia | TGCACGAGCGCTGAAGAA | CTGCTTCAACGCCACCAAC | 59.97 | 60.01 | 292 | 106 |
100.00%
|
50.00%
|