| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Faecalicatena | AGCCGTGGAAATCGCCAA | TTCTCTGGTGGCAAGTCCG | 59.97 | 59.63 | 208 | 130 |
100.00%
|
100.00%
|
| Primer 2 | Faecalicatena | AGTTGTAAGCCAGCTCGCA | TCTCTCGGTACCGGAGCAA | 59.63 | 60.00 | 180 | 130 |
100.00%
|
100.00%
|
| Primer 3 | Faecalicatena | GCCGAAGATGCAGGACTGT | ACTTCTGCTGCCGTTGCT | 60.08 | 59.89 | 153 | 128 |
100.00%
|
100.00%
|
| Primer 4 | Faecalicatena | TGCCGAAGATGCAGGACTG | ACTTCTGCTGCCGTTGCT | 60.08 | 59.89 | 154 | 128 |
100.00%
|
100.00%
|
| Primer 5 | Faecalicatena | TGCCGAAGATGCAGGACT | ACTTCTGCTGCCGTTGCT | 58.61 | 59.89 | 154 | 128 |
100.00%
|
100.00%
|
| Primer 6 | Faecalicatena | GAAGTCGGAGCAGACGTGA | ACTTCTGCTGCCGTTGCT | 59.42 | 59.89 | 203 | 128 |
100.00%
|
66.67%
|
| Primer 7 | Faecalicatena | AGAAGTCGGAGCAGACGTG | ACTTCTGCTGCCGTTGCT | 59.42 | 59.89 | 204 | 128 |
100.00%
|
66.67%
|
| Primer 8 | Faecalicatena | AATCAACGGTCCTGCGCT | CGGAGCATCTCTTCCGCTT | 59.65 | 59.86 | 265 | 125 |
100.00%
|
66.67%
|
| Primer 9 | Faecalicatena | GTTGTAAGCCAGCTCGCA | TCTCTCGGTACCGGAGCAA | 58.03 | 60.00 | 179 | 119 |
100.00%
|
100.00%
|
| Primer 10 | Faecalicatena | GCAGCAATCGAAGCAGCA | TCGCCAAGTCTTCCTGCTG | 59.13 | 60.00 | 164 | 116 |
100.00%
|
100.00%
|