| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Flaviflexus | TCGTTGATGCCACGGATCC | GCAACCGCTTCACCAAAGG | 60.15 | 60.01 | 193 | 100 |
100.00%
|
100.00%
|
| Primer 2 | Flaviflexus | GTGAAACGGATCCGCATGG | AACCGTCAAGGCTCTCCAC | 59.28 | 59.63 | 208 | 92 |
100.00%
|
100.00%
|
| Primer 3 | Flaviflexus | ACTCATCGCTGGGCTTTCC | ACTCCGCTACGTTTCCTGC | 60.08 | 60.08 | 248 | 91 |
100.00%
|
100.00%
|
| Primer 4 | Flaviflexus | TCCGCATGTGGATCAGCAG | ACTCCGCTACGTTTCCTGC | 60.15 | 60.08 | 232 | 91 |
100.00%
|
100.00%
|
| Primer 5 | Flaviflexus | AGCATTCGCCATCGCCAT | AGTGCATATGCCAGCGAGG | 60.20 | 60.23 | 240 | 90 |
100.00%
|
100.00%
|
| Primer 6 | Flaviflexus | TCGGCAGAGCTCAAACAGG | TGTGGTCAGTCGCGTTGT | 60.00 | 59.50 | 241 | 89 |
100.00%
|
100.00%
|
| Primer 7 | Flaviflexus | TCGACATCGGTGGGGAAAC | TGTGGTCAGTCGCGTTGT | 59.71 | 59.50 | 180 | 89 |
100.00%
|
100.00%
|
| Primer 8 | Flaviflexus | TCGTGCCGTTCATCGTGT | ACCGAGATCACGACCGAAC | 59.66 | 59.49 | 220 | 89 |
100.00%
|
100.00%
|
| Primer 9 | Flaviflexus | TCACCGTATCTGCCGATGC | ATCATCGTCGTGCGCTGT | 59.93 | 59.82 | 211 | 84 |
100.00%
|
100.00%
|
| Primer 10 | Flaviflexus | TACGCAAACAGGTCAGGGC | TTTCATGCCCAGCCGTGT | 60.30 | 59.88 | 232 | 84 |
100.00%
|
100.00%
|