| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Flaviflexus | TGCACCTTCGCTGTGACA | AAGGCTGATCAGTGCGCA | 59.50 | 59.65 | 267 | 78 |
100.00%
|
100.00%
|
| Primer 42 | Flaviflexus | AGGTTGAGGCCTCGCAAA | TGAGCATGTCGCCAAGCA | 59.16 | 59.97 | 276 | 78 |
100.00%
|
100.00%
|
| Primer 43 | Flaviflexus | AAGGTTGAGGCCTCGCAAA | TGAGCATGTCGCCAAGCA | 59.85 | 59.97 | 277 | 78 |
100.00%
|
100.00%
|
| Primer 44 | Flaviflexus | ACGTGTCCTCACCGTTGAC | CGAGGGCAACGTTGGAGAT | 59.93 | 60.08 | 155 | 77 |
100.00%
|
100.00%
|
| Primer 45 | Flaviflexus | AATACTTGGCTCGCCTCCG | TGCAGCATCAACCACGCA | 59.86 | 60.59 | 179 | 76 |
100.00%
|
100.00%
|
| Primer 46 | Flaviflexus | CGATGATGGACGTGTCGGA | TTCGTTGACGGTGCCGAT | 59.57 | 59.66 | 221 | 76 |
100.00%
|
100.00%
|
| Primer 47 | Flaviflexus | ATCGCGATGCACACGGAT | TCATCGTGCCATGGATCCG | 59.89 | 59.93 | 160 | 76 |
100.00%
|
100.00%
|
| Primer 48 | Flaviflexus | CACACCCAACGCTGGTTTG | GGATGCTCGAATGCCGTTG | 59.93 | 59.65 | 167 | 76 |
100.00%
|
100.00%
|
| Primer 49 | Flaviflexus | CACACCCAACGCTGGTTTG | TCGGGTGATCATCGGATGC | 59.93 | 59.63 | 180 | 76 |
100.00%
|
100.00%
|
| Primer 50 | Flaviflexus | AAACCGACGCTCATGCCA | AGGCAAGCAGAATCCGGTC | 59.97 | 60.08 | 279 | 76 |
100.00%
|
100.00%
|