| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Francisella | TGGTGGTGGATGGTGCTT | TCCAGCGCTATCACCCAT | 58.74 | 58.03 | 229 | 69 |
100.00%
|
100.00%
|
| Primer 252 | Francisella | ACATGGTGAAGCGTGGCT | TTTCGCGTGCCCAGTCTT | 59.57 | 59.89 | 275 | 69 |
100.00%
|
50.00%
|
| Primer 253 | Francisella | TGCAGCAACACGCAAGCT | TTTCGCGTGCCCAGTCTT | 61.21 | 59.89 | 200 | 69 |
100.00%
|
50.00%
|
| Primer 254 | Francisella | TCAATCCAGCAGCTGATGTAC | ACCATCTGCCCACCTTCTT | 58.09 | 58.52 | 190 | 69 |
100.00%
|
50.00%
|
| Primer 255 | Francisella | CAATCCAGCAGCTGATGTACA | AACCATCTGCCCACCTTCTT | 58.36 | 59.22 | 190 | 69 |
100.00%
|
50.00%
|
| Primer 256 | Francisella | TGCAAGATTATCACAGGCGAA | ACCATCTGCCCACCTTCTT | 58.02 | 58.52 | 263 | 69 |
100.00%
|
50.00%
|
| Primer 257 | Francisella | TCAACCAATCCGCAGATAGC | CTTGAGCAGTGCGTCCAGA | 58.05 | 60.01 | 151 | 69 |
100.00%
|
50.00%
|
| Primer 258 | Francisella | CGCTCAACTTGCAACGCTT | ACACCACCTTCGCCATGA | 60.01 | 58.84 | 201 | 69 |
100.00%
|
50.00%
|
| Primer 259 | Francisella | TCAGGCTTTCAAAGGTTTCGT | CAGCCCTACCAGTAAGACCT | 58.35 | 58.13 | 203 | 68 |
100.00%
|
50.00%
|
| Primer 260 | Francisella | TCAGGCTTTCAAAGGTTTCGT | GCCCTACCAGTAAGACCTCTC | 58.35 | 58.97 | 201 | 68 |
100.00%
|
50.00%
|