| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Frankia | ATCCGTCGTGGTGGAAAGG | AACGGCACTTCATCGGCA | 59.70 | 59.97 | 220 | 128 |
100.00%
|
50.00%
|
| Primer 2 | Frankia | ACATCCGTCGTGGTGGAAA | AACGGCACTTCATCGGCA | 59.25 | 59.97 | 222 | 127 |
100.00%
|
50.00%
|
| Primer 3 | Frankia | AGCTGCTGCGCTTCTTCA | GCGCATCGTGTGGATCAT | 59.97 | 58.28 | 173 | 122 |
100.00%
|
50.00%
|
| Primer 4 | Frankia | CACATCCGTCGTGGTGGAA | AACGGCACTTCATCGGCA | 60.01 | 59.97 | 223 | 118 |
100.00%
|
50.00%
|
| Primer 5 | Frankia | ACATCCGTCGTGGTGGAA | AACGGCACTTCATCGGCA | 58.54 | 59.97 | 222 | 118 |
100.00%
|
50.00%
|
| Primer 6 | Frankia | CGTCGTGGTGGAAAGGTCT | AACGGCACTTCATCGGCA | 59.63 | 59.97 | 217 | 117 |
100.00%
|
50.00%
|
| Primer 7 | Frankia | TCAAGCGCAACCTCACCA | ATGACACCCTTGCTGACGG | 59.49 | 60.00 | 178 | 90 |
100.00%
|
50.00%
|
| Primer 8 | Frankia | TCAAGCGCAACCTCACCA | GCGTGAATGACACCCTTGC | 59.49 | 59.79 | 184 | 75 |
100.00%
|
50.00%
|
| Primer 9 | Frankia | AGCTTGACGGCACCAAGA | TGCGGTGAACTCGTAGGTC | 59.17 | 59.42 | 281 | 75 |
100.00%
|
50.00%
|
| Primer 10 | Frankia | TCCGACGCACGTATCAGTG | TTGGTCGTGTAGTCACCGC | 59.86 | 60.01 | 185 | 75 |
100.00%
|
50.00%
|